Protein Desalting Products

In proteomics and mass spectrometry workflows, residual salts, detergents, reducing agents, and other low-molecular-weight contaminants can severely interfere with chromatographic separation and mass spectrometric ionization, resulting in decreased analytical sensitivity, elevated background noise, and signal suppression. Efficient desalting and concentration constitute critical sample preparation steps for acquiring high-quality mass spectrometry data. MtoZ Biolabs offers a comprehensive portfolio ranging from classical dialysis systems to high-speed centrifugal desalting columns and high-throughput filtration plates, enabling rapid and gentle removal of diverse interfering substances while supporting flexible sample concentration and buffer exchange, thereby ensuring sample purity for downstream LC-MS/MS analysis.

Products

Dialysis Products

Based on semipermeable membrane-mediated selective diffusion, these products provide classical and gentle desalting and buffer exchange solutions for sample volumes of different scales, and are particularly suitable for shear-sensitive proteins or samples requiring extended equilibration.

Desalting Centrifugal Columns

Leveraging size-exclusion chromatography principles, these columns achieve rapid desalting and buffer exchange of small-volume samples within minutes under high-speed centrifugation. The workflow is straightforward and exhibits high sample recovery, making the columns an ideal option for post-digestion peptide samples or limited protein quantities.

Filter Plates

Implemented in a 96-well plate format to support high-throughput processing, these filtration devices enable simultaneous desalting, clarification, or concentration of large sample batches, substantially improving sample preparation efficiency and meeting the requirements of high-throughput screening in proteomics.

Protein Concentration Tubes

Using centrifugal ultrafiltration, membranes with different molecular weight cut-offs (MWCO) are selected according to protein or peptide size, allowing efficient removal of low-molecular-weight contaminants while concentrating dilute samples, thus accomplishing concentration together with desalting or buffer exchange in a single step.

Lab Equipment at MtoZ Biolabs

Refrigerated Centrifuge

Low-temperature centrifugation prevents protein degradation and preserves the integrity of protein complexes and post-translational modifications, ensuring that enriched materials maintain authentic biological activity for downstream analyses.

High-Speed Microcentrifuge

Enables efficient centrifugation for Protein Desalting Products, centrifugal concentration devices, and related sample preparation consumables.

Vortex Mixer

Provides efficient yet gentle sample agitation, improving binding performance and reproducibility during immunoprecipitation or affinity enrichment workflows, while maintaining operational simplicity and throughput.

Vacuum Concentrator

Low-temperature vacuum concentration minimizes peptide degradation and modification loss, supports high sample throughput, and enhances overall mass-spectrometry pre-processing efficiency.

Advantages

Efficient Removal of Mass Spectrometry Interferences

MtoZ Biolabs Protein Desalting Products rapidly eliminate SDS, Triton X-100, urea, salts (e.g., NaCl, (NH4)2HCO3), DTT, and other major interfering substances, thereby improving spectral quality and signal-to-noise performance in LC-MS/MS analyses.

High Sample Recovery

Optimized membrane materials and device architectures ensure high recovery rates with minimal adsorption loss of proteins or digested peptides, making the system particularly suitable for precious or limited clinical and research samples.

Rapid and Streamlined Operation

Most formats utilize centrifugation or vacuum-based workflows with standardized protocols and do not require specialized instrumentation, enabling rapid batch processing and substantially improving laboratory efficiency.

Broad Sample Compatibility

The product line supports desalting of intact proteins and digested peptides across volume ranges from several microliters to multiple milliliters, offering flexible options for diverse experimental needs.

Technical Support

Protocol Downloads

MtoZ Biolabs provides comprehensive experimental protocols and product manuals to help ensure smooth and reproducible workflows. For access to specific documents or detailed instructions, please contact our support team.

Customized Service Support

Our experienced scientists offer tailored technical assistance and customized solutions based on your specific research goals, helping you achieve optimal experimental outcomes.

FAQ and Expert Assistance

We provide responsive and professional technical support to address your questions efficiently. Our experts are ready to help you identify and resolve experimental challenges quickly and accurately.