Proteolytic Enzymes

MtoZ Biolabs Proteolytic Enzymes (MS-Grade): MS-Grade Proteases With Ultra-High Purity and Substrate Specificity That Support Deep Proteomic Profiling and High-Accuracy Mass Spectrometry-Based Protein Identification

Proteases serve as essential tools in proteomic workflows, and their quality directly influences the generation of peptide products suitable for MS analysis. Impurities, autolytic fragments, or non-specific cleavage events present in non-MS-grade enzymatic preparations introduce substantial background signals that can interfere with MS-based protein identification, ultimately reducing protein sequence coverage and increasing false-positive identifications. The MS-Grade Protease Series from MtoZ Biolabs undergoes extensive purification and rigorous quality control, exhibiting near-zero autolytic activity and high cleavage specificity. These properties enable efficient and accurate proteolytic conversion of protein samples into high-quality peptide mixtures, providing well-defined inputs for downstream LC-MS/MS-based identification, quantitative analysis, and post-translational modification characterization.

 

Advantages

High Purity and Cleavage Specificity

MtoZ Biolabs Proteolytic Enzymes are manufactured through advanced purification workflows to eliminate contaminating proteases and autolytic fragments. Each enzyme exhibits defined cleavage specificity (e.g., Trypsin cleaves C-terminal to K/R, Lys-C cleaves C-terminal to K), thereby minimizing off-target cleavage events and generating more uniform and predictable peptide products.

Minimal Autolysis Background

MS-grade preparation minimizes autolytic degradation of the enzyme itself, resulting in negligible enzyme-derived peptide signals in mass spectra. This substantially reduces analytical background and improves signal-to-noise ratio and detection sensitivity for target protein identification.

Consistent Performance Across Batches

Rigorous activity unit (U) calibration and stability testing ensure that different production batches maintain equivalent digestion efficiency and performance, enabling highly comparable and reproducible datasets for long-term studies or large-scale projects.

Extensive Workflow Compatibility

The digestion buffer is compatible with common denaturants (e.g., urea) and supports multiple proteolysis workflows including in-solution, in-gel, and on-membrane digestion. MtoZ Biolabs provides multiple proteolytic enzymes and enzyme combinations to accommodate a broad spectrum of applications ranging from routine protein identification to deep proteome coverage, cross-linking mass spectrometry (XL-MS), and hydrogen–deuterium exchange mass spectrometry (HDX-MS).

Technical Support

Protocol Downloads

MtoZ Biolabs provides comprehensive experimental protocols and product manuals to help ensure smooth and reproducible workflows. For access to specific documents or detailed instructions, please contact our support team.

Customized Service Support

Our experienced scientists offer tailored technical assistance and customized solutions based on your specific research goals, helping you achieve optimal experimental outcomes.

FAQ and Expert Assistance

We provide responsive and professional technical support to address your questions efficiently. Our experts are ready to help you identify and resolve experimental challenges quickly and accurately.