The MtoZ Biolabs' MS Grade Glu-C Protease primarily cleaves the C-terminus of glutamic acid (Glu) and aspartic acid (Asp) residues in proteins. This protease helps advance LC-MS/MS protein identification and improves sequence coverage in proteomics research.
Product Overview
When performing protein sample digestion and mass spectrometry analysis, digestion efficiency, site specificity, and system compatibility often become limiting factors for data quality, mainly manifested in the following aspects:
1. Incomplete Digestion and Missed Cleavage
Dense structures or insufficient denaturation limit the exposure of cleavage sites, resulting in longer peptides and reduced identification rates.
2. Overdigestion and Nonspecific Cleavage
Unstable reaction conditions or inconsistent enzyme activity can lead to nonspecific peptides, interfering with database search and quantitative consistency.
3. Matrix Inhibition
Residual serum, tissue lysates, or high-salt detergents can inhibit enzyme activity and increase background, reducing batch-to-batch reproducibility.
4. Uneven Peptide Distribution
Relying solely on trypsin can lead to biased peptide coverage, making it difficult to complete sequence information for certain regions.
5. Downstream Compatibility
Enzyme preparations containing impurities or autolytic fragments can increase MS background and affect the detection and quantification stability of low-abundance proteins.
Advantages
✅ Increased Sequence Coverage
By specifically cleaving at Glu-C sites, MtoZ Biolabs' MS Grade Glu-C Protease complements the trypsin peptide map, enhancing sequence coverage and site-specific analysis.
✅ High Specific Activity
The high enzymatic activity ensures more controlled digestion efficiency and stable peptide yields, improving identification and quantification consistency.
✅ Stable
Stable enzymatic performance reduces batch-to-batch variability and condition sensitivity, making it compatible with common denaturation conditions and buffers, while minimizing experimental fluctuations.
Technical Support
1. Protocol Downloads
MtoZ Biolabs provides comprehensive experimental protocols and product manuals to help ensure smooth and reproducible workflows. For access to specific documents or detailed instructions, please contact our support team.
2. Customized Service Support
Our experienced scientists offer tailored technical assistance and customized solutions based on your specific research goals, helping you achieve optimal experimental outcomes.
3. FAQ and Expert Assistance
We provide responsive and professional technical support to address your questions efficiently. Our experts are ready to help you identify and resolve experimental challenges quickly and accurately.
