Activity-based chemical proteomics represents a powerful approach for the systematic interrogation of enzyme function, the identification of druggable targets, and the assessment of inhibitor potency. The product portfolio of MtoZ Biolabs offers activity site-directed labeling and enrichment solutions targeting major enzyme families, including kinases, GTPases, and serine hydrolases, based on ActivX probes. These probes selectively and covalently engage the active sites of target enzymes, thereby enabling the enrichment and analysis of highly specific enzyme subproteomes. This strategy provides a robust platform for investigating the regulation of enzymatic activity, conducting inhibitor screening, and performing target validation.
Products
Enzyme Enrichment Kits and Probes
Based on broad-spectrum or selective ATP/ADP analog probes, these reagents enable the covalent labeling and enrichment of active kinases. They are widely applied in global kinome activity profiling, kinase inhibitor target identification, and the evaluation of inhibitor selectivity.
GTPase Enrichment Kits with GTP Probes
Utilizing activated GTP analog probes, these kits specifically label and capture GTPase family proteins, such as Ras and Rho, that exhibit GTP-binding activity. They serve as powerful tools for investigating the regulation of GTPase activity and pharmacological modulation within signal transduction pathways.
Serine Hydrolase Probes
These probes employ covalent labeling strategies targeting the active-site serine residue, allowing for the selective enrichment of serine hydrolase family enzymes, including proteases, esterases, and lipases. They are well suited for functional annotation, activity-based profiling, and inhibitor discovery within this enzyme family.
Lab Equipment at MtoZ Biolabs
Refrigerated Centrifuge
Continuous low-temperature operation effectively prevents protein degradation while preserving the integrity of protein complexes and post-translational modifications, thereby ensuring the biological activity and reliability of enriched samples.
Vortex Mixer
Enables efficient yet gentle mixing, markedly enhancing the binding efficiency and reproducibility of immunoprecipitation or affinity-based enrichment procedures, while offering straightforward and time-efficient operation.
Vacuum Concentrator
A mild low-temperature concentration process minimizes degradation of target peptides and prevents the loss of labile modifications. Its high-throughput capability significantly improves the efficiency of mass spectrometry sample preparation.
Streptavidin Magnetic Bead Separation System
Designed for the efficient capture and isolation of biotin-labeled enzyme-probe complexes.
Advantages
High Specificity and Activity-Oriented Design
The probes are designed based on native enzyme substrates or catalytic mechanisms, enabling selective covalent labeling of functional enzymes with intact active sites. As a result, the labeling signal directly reports enzymatic activity rather than protein abundance.
A Robust Chemical Proteomics Approach
Through conjugation with biotin or reporter tags, the activity-defined subpopulation of target enzyme families can be efficiently enriched and purified from complex biological samples, followed by downstream identification by mass spectrometry. This approach substantially streamlines activity-based target discovery.
Broad Applicability
This platform is well suited for assessing intracellular target engagement of small-molecule inhibitors, determining inhibitor binding potency (IC50), and performing competitive binding assays, thereby facilitating drug screening and optimization.
Excellent Compatibility with Mass Spectrometry
Optimized probe structures and experimental workflows ensure that enriched samples are readily amenable to liquid chromatography-tandem mass spectrometry (LC-MS/MS) analysis, enabling high-confidence protein identification.
Technical Support
Protocol Downloads
MtoZ Biolabs provides comprehensive experimental protocols and product manuals to help ensure smooth and reproducible workflows. For access to specific documents or detailed instructions, please contact our support team.
Customized Service Support
Our experienced scientists offer tailored technical assistance and customized solutions based on your specific research goals, helping you achieve optimal experimental outcomes.
FAQ and Expert Assistance
We provide responsive and professional technical support to address your questions efficiently. Our experts are ready to help you identify and resolve experimental challenges quickly and accurately.
- Serine Hydrolase ProbesMtoZ Biolabs Serine Hydrolase Probes: Broad-Spectrum Capture of Active Serine Hydrolases and Investigation of Functional and Target Roles in Metabolic, Neurological, and Immune Regulation Serine hydrolases constitute a large enzyme superfamily that includes proteases such as trypsin and thrombin, as well as esterases, lipases, and amidases. These enzymes participate in diverse biological processes, including metabolism, digestion, neuronal signaling, immune responses, and apoptosis. Designed around t......
- GTPase Enrichment Kits with GTP ProbesMtoZ Biolabs GTPase Enrichment Kits with GTP Probes - Precise Capture of GTPase Active States and Dissection of Signaling and Disease Mechanisms GTPases function as key molecular switches that regulate essential cellular processes such as proliferation, motility, and membrane trafficking. Their active states, defined by binding to either GTP or GDP, determine the activation status of signaling pathways. Built on activated GTP analog probes, the MtoZ Biolabs GTPase enrichment product line selectively ......
- Enzyme Enrichment Kits and ProbesKinases serve as central regulators of cellular signal transduction and constitute one of the most important classes of drug targets. Conventional approaches primarily quantify kinase abundance and fail to discriminate between inactive and active states. The kinase activity enrichment product portfolio developed by MtoZ Biolabs leverages innovative ActivX ATP/ADP analog probes to specifically and covalently label the active sites of kinases, thereby enabling the direct enrichment and identification of .....