MtoZ Biolabs' Peptide Retention Time Calibration Mixture, LC-MS Grade is an LC-MS-grade mixture of characteristic peptides designed for retention time calibration. It can be used to establish and correct RT baselines and to monitor chromatographic reproducibility. The mixture helps advance DDA and DIA method development, cross-batch data alignment, and proteomics QC analysis.
Product Overview
In high-throughput proteomics projects, small deviations in chromatography and data processing can quickly escalate into comparability issues. Key challenges include:
1. Retention Time Drift Leads to Alignment Failures
Column aging, gradient mixing errors, or temperature fluctuations can shift RT globally, making cross-run alignment and quantitative comparisons unstable.
2. Peak-Shape Changes Compromise Identification and Quantitation
Flow-path contamination, dead volume, or solvent changes can cause peak broadening, tailing, and co-elution, reducing peptide matching confidence and quantitative consistency.
3. Limited Cross-Batch and Cross-Platform Comparability
Differences in columns, systems, or day-to-day operating conditions can distort the RT scale, affecting library matching, DIA extraction windows, and result reproducibility.
4. High Trial-and-Error Cost in Library Building and Method Development
Without a standardized RT ruler, gradient length, separation windows, and iRT/RT models often require repeated tuning, consuming instrument time and extending project timelines.
5. Automated Data Processing Depends on a Stable RT Model
DIA analysis, library searching, and machine-learning scoring are sensitive to RT prediction and correction. An unstable RT reference increases the risk of false matches and missed identifications.
Advantages
✅Standardized RT Ruler: Provides characteristic peptides spanning a wide hydrophobicity range for robust RT calibration curves and iRT/RT mapping.
✅Improved Alignment and Comparability: Helps unify RT scales across batches, gradients, and platforms, reducing alignment error.
✅Compatible: Supports spectral library generation, DIA extraction window setup, RT prediction model calibration, and system suitability evaluation.
✅High Consistency and Reproducibility: Stable composition and performance enable long-term trending of chromatographic health and method drift.
✅Low Background: Tight control of impurities and background signals minimizes interfering peaks that can impact RT and peak-shape assessment.
Technical Support
1. Protocol Downloads
MtoZ Biolabs provides comprehensive experimental protocols and product manuals to help ensure smooth and reproducible workflows. For access to specific documents or detailed instructions, please contact our support team.
2. Customized Service Support
Our experienced scientists offer tailored technical assistance and customized solutions based on your specific research goals, helping you achieve optimal experimental outcomes.
3. FAQ and Expert Assistance
We provide responsive and professional technical support to address your questions efficiently. Our experts are ready to help you identify and resolve experimental challenges quickly and accurately.
