DSG (Disuccinimidyl Glutarate) from MtoZ Biolabs is a high-purity, amine-reactive homobifunctional crosslinker designed for covalent stabilization of protein-protein interactions and biomolecular conjugation under controlled experimental conditions.
Product Overview
In protein interaction and conjugation studies, crosslinker design directly influences interaction capture and downstream analysis. Among commonly available crosslinking reagents, several inherent limitations are often encountered:
1. Spacer Length Not Suitable for Short-Range Interactions
Crosslinkers with excessively long or poorly defined spacer arms may fail to accurately stabilize closely associated protein interactions.
2. Inconsistent Amine Reactivity
Variability in NHS ester stability and reagent purity can lead to uneven reactivity toward lysine residues and protein N-termini, affecting reproducibility.
3. Structural Perturbation of Protein Complexes
Some crosslinkers introduce unnecessary structural constraints, complicating interpretation in interaction mapping or structural studies.
4. Limited Downstream Compatibility
Certain chemical properties may interfere with electrophoresis, immunodetection, or mass spectrometry-based workflows.
Advantages
The MtoZ Biolabs DSG (Disuccinimidyl Glutarate) offers the following benefits:
✅ Defined Spacer Length for Precise Interaction Capture
Our DSG provides a well-characterized spacer suitable for stabilizing protein-protein interactions at short to intermediate distances.
✅ Reliable NHS Ester Chemistry
Efficient and specific reactivity with primary amines supports consistent covalent crosslink formation.
✅ Support for Native Interaction States
Compatible with mild reaction conditions, helping preserve protein conformation and interaction specificity.
✅ Compatibility With Standard Analytical Workflows
Crosslinked samples are suitable for SDS-PAGE, immunoblotting, and proteomics-based analysis.
Technical Support
1. Protocol Downloads
MtoZ Biolabs provides comprehensive experimental protocols and product manuals to help ensure smooth and reproducible workflows. For access to specific documents or detailed instructions, please contact our support team.
2. Customized Service Support
Our experienced scientists offer tailored technical assistance and customized solutions based on your specific research goals, helping you achieve optimal experimental outcomes.
3. FAQ and Expert Assistance
We provide responsive and professional technical support to address your questions efficiently. Our experts are ready to help you identify and resolve experimental challenges quickly and accurately.
