The Azide-FP Serine Hydrolase Probe from MtoZ Biolabs is an activity-based labeling reagent that covalently tags active serine hydrolases for downstream click chemistry enrichment and profiling by Western blot or LC-MS.
Product Overview
Serine hydrolases form a large and diverse superfamily, so functional studies often face practical bottlenecks such as:
1. Active vs Inactive Ambiguity
Conventional readouts struggle to distinguish active enzymes from zymogens, inactive forms, or proteins that are present but not catalytically engaged.
2. Missing a Global Activity View
Complex samples make it hard to profile hundreds of serine hydrolases in an unbiased, class wide manner within a single experiment.
3. Slow Inhibitor Characterization
Selectivity and potency testing is often performed one target at a time, limiting throughput and making off target effects easy to miss.
4. Fragmented Downstream Readouts
Activity labeling and deep target identification are frequently handled in separate workflows, leading to extra handling and incomplete information.
Advantages
✔️Catalytic Site Covalent Labeling
The FP warhead selectively reacts with the catalytic serine in active sites, providing a direct activity readout rather than abundance.
✔️Click Chemistry Flexibility
The azide handle supports rapid conjugation to biotin, fluorophores, or other reporters, enabling clean enrichment or in-gel detection when needed.
✔️Competition Based Inhibitor Profiling
Co incubation with candidate small molecules enables competition experiments in complex matrices such as cell lysates, tissue homogenates, or purified proteins, supporting quantitative readouts of inhibitor engagement and selectivity across serine hydrolases.
✔️Downstream Analysis Compatibility
MtoZ Biolabs' Azide-FP Serine Hydrolase Probe supports in gel profiling after enrichment for rapid verification and can be coupled to LC-MS/MS after digestion to enable broad, unbiased identification of active enzymes and potential off targets.
Technical Support
1. Protocol Downloads
MtoZ Biolabs provides comprehensive experimental protocols and product manuals to help ensure smooth and reproducible workflows. For access to specific documents or detailed instructions, please contact our support team.
2. Customized Service Support
Our experienced scientists offer tailored technical assistance and customized solutions based on your specific research goals, helping you achieve optimal experimental outcomes.
3. FAQ and Expert Assistance
We provide responsive and professional technical support to address your questions efficiently. Our experts are ready to help you identify and resolve experimental challenges quickly and accurately.
