MtoZ Biolabs' Bovine Immunoglobulin G (IgG) ELISA Kit is a high-specificity immunoassay kit designed for the quantitative detection of bovine IgG. It accurately measures IgG levels in serum, plasma, colostrum, milk, and other bovine-derived biological samples. This kit is widely used in passive immunity assessment, colostrum quality evaluation, veterinary disease monitoring, and bovine-derived raw material quality control.
Product Overview
Bovine IgG is the most abundant immunoglobulin in cattle serum and the primary antibody transferred via colostrum to neonatal calves. Accurate quantification is critical for immune status evaluation, yet presents several challenges:
1. Extremely Wide Concentration Range
Bovine IgG levels vary from mg/mL in serum and colostrum to µg/mL or lower in milk and processed dairy products, demanding broad dynamic range coverage.
2. Cross-Reactivity with Other Species
Structural homology between bovine IgG and immunoglobulins from other ruminant species (sheep, goat) or other bovine isotypes (IgM, IgA) increases the risk of non-specific detection.
3. Complex Sample Matrices
Colostrum and milk contain high concentrations of fats, casein, and lactoferrin that can interfere with antigen-antibody interactions and signal readout.
4. Subclass Variability
Bovine IgG comprises two major subclasses ( IgG1 and IgG2) with distinct structural and functional properties, requiring antibodies capable of recognizing both subclasses for total IgG quantification.
5. Post-Collection Stability
IgG in colostrum and milk samples is susceptible to degradation by endogenous proteases and bacterial contamination, requiring careful sample handling to preserve analyte integrity.
Advantages
✅ Sensitivity
LOD reaches the ng/mL level.
✅ Specificity
Antibodies target epitopes unique to bovine IgG heavy chain (γ chain), with minimal cross-reactivity to bovine IgM, IgA, or immunoglobulins from other species.
✅ Precision
Intra-assay and inter-assay CVs are within acceptable limits.
Technical Support
1. Protocol Downloads
MtoZ Biolabs provides comprehensive experimental protocols and product manuals to help ensure smooth and reproducible workflows. For access to specific documents or detailed instructions, please contact our support team.
2. Customized Service Support
Our experienced scientists offer tailored technical assistance and customized solutions based on your specific research goals, helping you achieve optimal experimental outcomes.
3. FAQ and Expert Assistance
We provide responsive and professional technical support to address your questions efficiently. Our experts are ready to help you identify and resolve experimental challenges quickly and accurately.
