Protein Interaction and Crosslinking Reagents

Understanding protein interaction networks and deciphering the structure and dynamics of protein complexes are essential for elucidating the molecular mechanisms that underlie cellular processes. Traditional approaches such as co-immunoprecipitation (Co-IP) often fail to capture transient or weak interactions and lack the ability to reveal structural interface information. The MtoZ Biolabs Protein Interaction and Crosslinking Reagents provide a comprehensive toolkit for probing protein-protein interactions with enhanced structural resolution. Leveraging chemical crosslinking under near-physiological conditions and high-resolution mass spectrometry, these solutions enable covalent locking of interacting proteins, precise identification of interacting partners, and mapping of binding interfaces, thereby accelerating the generation of high-confidence protein interaction landscapes.

Products

Crosslinking Reagents

MtoZ Biolabs offers a diverse selection of Crosslinking Reagents designed to support flexible and application-specific experimental workflows. Available formats include homobifunctional and heterobifunctional reagents, membrane-permeable crosslinkers, adjustable spacer arm lengths, and affinity-capture tag options. These reagents enable rational crosslinking strategy design for applications such as in-cell in situ crosslinking and solution-phase protein complex crosslinking, supporting exploratory studies across a broad range of sample types.

Protein-Protein Crosslinking Kits

The Protein-Protein Crosslinking Kits provide standardized, ready-to-use workflows for XL-MS applications. Each kit includes optimized crosslinkers, quenching reagents, digestion and enrichment components, and validated protocols. The integrated kit format minimizes hands-on complexity, improves reproducibility, and streamlines the preparation of crosslinked protein complexes for downstream mass spectrometry. These kits are an ideal solution for researchers conducting comprehensive interaction proteomics at scale.

Lab Equipment at MtoZ Biolabs

Refrigerated Centrifuge

It enables continuous low-temperature sample processing to prevent protein degradation and preserve the structural integrity of protein complexes and post-translational modifications. This ensures that enriched samples retain their biological relevance and authenticity for subsequent analysis.

High-Performance Liquid Chromatography (HPLC) System

The HPLC System is used for efficient separation of crosslinked peptides, increasing mass spectrometry identification depth, and expanding proteome coverage within crosslinking workflows.

Vortex Mixer

The Vortex Mixer provides efficient yet gentle mixing, significantly improving binding efficiency and consistency in immunoprecipitation and affinity enrichment workflows. The system is simple to operate and helps reduce hands-on time for the user.

Vacuum Concentrator

It supports low-temperature sample concentration, minimizing peptide degradation and preventing the loss of labile modifications. Its high-throughput design accelerates sample preparation and greatly enhances pre-MS processing efficiency.

Advantages

Capture Transient and Weak Interactions

Chemical crosslinking preserves transient or low-affinity interactions that are difficult to detect using traditional biochemical techniques, enabling more realistic interaction mapping in biological systems.

Reveal Interaction Interfaces

XL-MS data provides direct structural insights by identifying specific crosslinking sites, offering valuable clues for mechanistic studies and structure-guided drug discovery.

Compatible with Complex and Physiological Systems

Select crosslinkers support in-cell in situ applications, enabling analysis under near-physiological conditions. When combined with subcellular fractionation, researchers can dissect interaction networks with organelle-level resolution.

High Sensitivity and Confidence

Optimized enrichment approaches and advanced data analysis pipelines allow for the detection of low-abundance crosslinked peptides from complex backgrounds with stringent validation criteria to ensure data confidence.

Technical Support

Protocol Downloads

MtoZ Biolabs provides comprehensive experimental protocols and product manuals to help ensure smooth and reproducible workflows. For access to specific documents or detailed instructions, please contact our support team.

Customized Service Support

Our experienced scientists offer tailored technical assistance and customized solutions based on your specific research goals, helping you achieve optimal experimental outcomes.

FAQ and Expert Assistance

We provide responsive and professional technical support to address your questions efficiently. Our experts are ready to help you identify and resolve experimental challenges quickly and accurately.