DSP (dithiobis(succinimidyl propionate)) from MtoZ Biolabs is a thiol-cleavable, amine-reactive homobifunctional crosslinker designed for reversible stabilization of protein-protein interactions and controlled downstream analysis.
Product Overview
In protein interaction and complex analysis workflows, crosslinker chemistry and product consistency directly influence experimental reliability. Among commonly available amine-reactive crosslinkers, several inherent considerations are frequently encountered:
1. Lack of Controlled Reversibility
Non-cleavable linkers permanently fix protein complexes, limiting flexibility in workflows that require subsequent dissociation or detailed component analysis.
2. Inconsistent Chemical Purity
Variability in reagent purity and identity across products or batches can lead to unpredictable crosslinking efficiency and reduced reproducibility.
3. Limited Transparency in Manufacturing Quality
Insufficient characterization or batch-level documentation may complicate method development, validation, or long-term process consistency.
4. Challenges in Downstream Compatibility
Crosslinkers without a defined cleavage mechanism can restrict compatibility with reducing conditions commonly used in electrophoretic, immunological, or proteomics-based analyses.
Advantages
The MtoZ Biolabs DSP (dithiobis(succinimidyl propionate)) offers the following advantages:
✅ Thiol-Cleavable Disulfide Spacer
DSP incorporates a central disulfide bond that can be selectively cleaved under reducing conditions, enabling reversible stabilization and controlled release of protein complexes.
✅ Reliable Amine-Reactive NHS Ester Chemistry
Homobifunctional NHS ester groups react specifically with primary amines, including lysine side chains and protein N-termini, supporting efficient and predictable crosslink formation.
✅ High Level of Product Characterization
Each production lot undergoes rigorous identity and purity verification using multiple analytical approaches, supporting consistent experimental performance.
✅ Manufacturing Consistency and Traceability
Batch-specific documentation, retained reference lots, and formal change management practices support long-term reproducibility and method continuity.
✅ Compatibility With Standard Analytical Workflows
DSP-crosslinked samples can be readily processed under reducing conditions for SDS-PAGE, immunoblotting, immunoprecipitation, and mass spectrometry-based analysis.
Technical Support
1. Protocol Downloads
MtoZ Biolabs provides comprehensive experimental protocols and product manuals to help ensure smooth and reproducible workflows. For access to specific documents or detailed instructions, please contact our support team.
2. Customized Service Support
Our experienced scientists offer tailored technical assistance and customized solutions based on your specific research goals, helping you achieve optimal experimental outcomes.
3. FAQ and Expert Assistance
We provide responsive and professional technical support to address your questions efficiently. Our experts are ready to help you identify and resolve experimental challenges quickly and accurately.
