MtoZ Biolabs' Endoplasmic Reticulum Protein Extraction Kit delivers high-performance, reliable reagents specifically optimized for the challenges of membrane-associated protein isolation in modern proteomics workflows. The kit is ideal for applications such as subcellular proteome profiling, transmembrane protein characterization, post-translational modification (PTM) analysis, and quality control studies in disease and drug response research.
Product Overview
The ER proteome is enriched in transmembrane and membrane-associated proteins, which present unique extraction challenges due to their high hydrophobicity, complex lipid environment, and dynamic post-translational modifications (PTMs):
1. Strong Interference from Hydrophobic Lipid Bilayers
Conventional lysis buffers often fail to simultaneously solubilize lipid membranes and release membrane-bound proteins, leading to low recovery.
2. Poor Solubility of Transmembrane Proteins
Multi-pass membrane proteins tend to aggregate and precipitate, complicating enzymatic digestion and MS/PTM site accessibility.
3. Low-Abundance PTMs Can Be Lost or Masked
Modifications such as lysine acetylation (Kac) and phosphorylation on ER-resident proteins are often substoichiometric and may be overshadowed by abundant structural components.
Advantages
✅ Efficient Dual Solubilization and Protein Recovery
A specially formulated buffer system disrupts membranes while maximizing the yield of hydrophobic proteins.
✅ Anti-Precipitation Design Improves Downstream Accessibility
MtoZ Biolabs' Endoplasmic Reticulum Protein Extraction Kit maintains solubility of transmembrane proteins to facilitate enzymatic digestion and site-level identification.
✅ Preserves Native PTM Distribution and Quantification
Compatible with mapping diverse PTM landscapes across organelle-enriched fractions with high fidelity.
Technical Support
1. Protocol Downloads
MtoZ Biolabs provides comprehensive experimental protocols and product manuals to help ensure smooth and reproducible workflows. For access to specific documents or detailed instructions, please contact our support team.
2. Customized Service Support
Our experienced scientists offer tailored technical assistance and customized solutions based on your specific research goals, helping you achieve optimal experimental outcomes.
3. FAQ and Expert Assistance
We provide responsive and professional technical support to address your questions efficiently. Our experts are ready to help you identify and resolve experimental challenges quickly and accurately.
