The MtoZ Biolabs' Golgi Apparatus Protein Extraction Kit is designed specifically for the membrane-rich and fragile Golgi apparatus to overcome challenges in organelle isolation and integrity loss, enabling high-purity Golgi protein preparation that supports membrane protein research and functional analysis.
Product Overview
The Golgi apparatus consists of stacked cisternal membranes and is tightly associated with the endoplasmic reticulum and vesicular trafficking systems. Its fragile structure and complex membrane composition make the isolation and purification of Golgi proteins particularly challenging:
1. Susceptible to Rupture and Contamination
The thin Golgi membrane easily breaks under mechanical or chemical disruption, causing cytosolic and ER proteins to mix into the preparation and reduce purity.
2. Close Association with Other Membrane-Bound Organelles
Because the Golgi is structurally and physically linked to the ER, vesicles, and membrane protein complexes with similar density, conventional centrifugation often struggles to separate it effectively.
3. Poor Solubility of Golgi Membrane Proteins
The Golgi is enriched with lipids and transmembrane proteins that tend to aggregate or precipitate during extraction, and standard lysis buffers often fail to solubilize them efficiently.
4. Protein Degradation Caused by Endogenous Enzymes
As seen in typical subcellular extraction workflows, endogenous proteases can rapidly degrade organelle proteins if processing is delayed, compromising downstream analysis.
Advantages
✅ Gentle and Selective Lysis System
An optimized buffer system maintains Golgi membrane integrity while enabling efficient disruption, improving organelle specificity during fractionation.
✅ High-Efficiency Membrane Protein Solubilization
Formulation in MtoZ Biolabs' Golgi Apparatus Protein Extraction Kit enhances lipid compatibility and transmembrane protein extraction, ensuring difficult membrane proteins are fully released and suitable for MS, WB, and other analyses.
✅ Reduced Cross-Contamination via Graded Extraction
A multi-step lysis and sequential centrifugation workflow significantly minimizes contamination from ER and cytosolic components.
✅ Built-In Protease Inhibition System
A stabilized protease inhibitor mix protects Golgi proteins throughout the extraction and separation process, improving overall sample integrity and data reliability.
Technical Support
1. Protocol Downloads
MtoZ Biolabs provides comprehensive experimental protocols and product manuals to help ensure smooth and reproducible workflows. For access to specific documents or detailed instructions, please contact our support team.
2. Customized Service Support
Our experienced scientists offer tailored technical assistance and customized solutions based on your specific research goals, helping you achieve optimal experimental outcomes.
3. FAQ and Expert Assistance
We provide responsive and professional technical support to address your questions efficiently. Our experts are ready to help you identify and resolve experimental challenges quickly and accurately.
