MtoZ Biolabs' Histone Extraction Kit enables rapid, high-yield isolation of core and linker histones from cultured cells and tissue samples for downstream epigenetics, chromatin biology, and proteomics analysis.
Product Overview
Routine whole cell or nuclear lysis is often insufficient for histone extraction, as histones are tightly bound to DNA and easily lose post-translational modification information. The main challenges of histone extraction include:
1. Strong DNA-Protein Interactions
Robust DNA-protein interactions can trap histones in highly viscous chromatin fragments, making separation difficult and reducing the amount of soluble histone available for antibody based or MS based assays.
2. Labile Post Translational Modifications
Histone acetylation, methylation, phosphorylation, and other PTMs are sensitive to deacetylases, phosphatases, and proteases activated during lysis, which can alter modification patterns and compromise epigenetic readouts.
3. High DNA Content and Sample Viscosity
Large amounts of genomic DNA released during chromatin disruption increase viscosity, complicate pipetting and sample handling, and can interfere with gel separation and LC-MS performance.
4. Contamination by Non Histone Nuclear Proteins
Conventional nuclear extraction often co isolates abundant non histone proteins, reducing histone purity and increasing background in Western blotting, ChIP related assays, and mass spectrometry.
Advantages
✔️Selective Enrichment of Histones
The Histone Extraction Kit from MtoZ Biolabs uses an optimized extraction chemistry that preferentially releases core and linker histones from chromatin while limiting co extraction of bulk non histone nuclear proteins, improving sample specificity for epigenetic studies.
✔️Protection of Histone PTM Patterns
A tailored inhibitor system helps suppress proteases and PTM modifying enzymes, allowing researchers to preserve native acetylation, methylation, phosphorylation, and other histone marks during extraction.
✔️Reduced DNA Viscosity and Cleaner Samples
The workflow of this kit is designed to minimize DNA driven viscosity and to reduce chromatin debris, yielding histone enriched fractions that are easier to pipette and better suited for SDS PAGE, Western blotting, and LC MS based histone profiling.
✔️Consistent, Study Ready Performance
MtoZ Biolabs formulates the Histone Extraction Kit with a clear and reproducible protocol, supporting reliable histone yield, purity, and data quality across different cell types, tissues, and experimental designs.
Technical Support
1. Protocol Downloads
MtoZ Biolabs provides comprehensive experimental protocols and product manuals to help ensure smooth and reproducible workflows. For access to specific documents or detailed instructions, please contact our support team.
2. Customized Service Support
Our experienced scientists offer tailored technical assistance and customized solutions based on your specific research goals, helping you achieve optimal experimental outcomes.
3. FAQ and Expert Assistance
We provide responsive and professional technical support to address your questions efficiently. Our experts are ready to help you identify and resolve experimental challenges quickly and accurately.
