MtoZ Biolabs' Tissue Active Protein Extraction Kit enables highly efficient extraction of tissue proteins under non-denaturing conditions while preserving native biological activity and molecular interactions. It effectively addresses common challenges encountered in the isolation of membrane proteins and the removal of interfering substances.
Product Overview
When biologically active proteins are required for applications such as Co-IP, pull-down assays, or enzymatic activity analysis, conventional strongly denaturing lysis methods disrupt protein structure and intermolecular interactions. In contrast, the extraction of functional proteins under non-denaturing conditions involves several major technical challenges:
1. Low Yield of Membrane Proteins
Mild nonionic detergents alone are insufficient to efficiently solubilize hydrophobic membrane proteins, resulting in substantial loss of membrane-associated species during native extraction.
2. Interference from Genomic DNA and Lipids
Upon tissue disruption, released genomic DNA and lipids cause substantial sample viscosity and reduced clarity in gentle non-denaturing buffers, which severely compromise the efficiency and reliability of downstream immunoprecipitation and related assays.
3. Instability of Protein Activity
Extended extraction procedures may fail to adequately suppress protease activity, leading to degradation and loss of native function.
Advantages
1. Enhanced Extraction Efficiency for Membrane Proteins
MtoZ Biolabs' Tissue Active Protein Extraction Kit incorporates a rationally optimized combination of zwitterionic and nonionic detergents that greatly improves membrane protein solubilization while preserving native conformation, resulting in a broader and more representative proteome in the final extract.
2. Effective Removal of Interfering Components and Improved Sample Quality
The optimized workflow minimizes viscosity induced by genomic DNA and lipids, producing a clear and easy-to-manipulate lysate that is directly applicable to high-purity assays such as Co-IP.
3. Comprehensive Preservation of Protein Activity
A mild lysis formulation, together with potent protease inhibitors, provides a protective environment that maintains native protein structures and interaction capacities throughout the extraction process.
Technical Support
1. Protocol Downloads
MtoZ Biolabs provides comprehensive experimental protocols and product manuals to help ensure smooth and reproducible workflows. For access to specific documents or detailed instructions, please contact our support team.
2. Customized Service Support
Our experienced scientists offer tailored technical assistance and customized solutions based on your specific research goals, helping you achieve optimal experimental outcomes.
3. FAQ and Expert Assistance
We provide responsive and professional technical support to address your questions efficiently. Our experts are ready to help you identify and resolve experimental challenges quickly and accurately.
