The Lymphatic Vessel Tissue Protein Extraction Kit from MtoZ Biolabs enables efficient extraction of high-quality total protein from delicate, lymph-filled lymphatic vessel tissue for downstream biochemical and proteomic analysis.
Product Overview
Extracting total protein from lymphatic vessels with standard lysis buffers is technically challenging due to their unique structure and composition. The main challenges include:
1. Fragile Thin-Walled Anatomy
Lymphatic vessels have very thin walls composed of endothelial cells and sparse smooth muscle, making them prone to tearing or loss during homogenization if lysis conditions are not well controlled.
2. Low Cellular Protein Content
Compared with solid organs, lymphatic vessels contain few cells per unit length, so conventional extraction methods often yield insufficient protein for parallel or quantitative assays.
3. Lymph-Borne Contaminants
Residual lymph carries lipids, immune proteins, and soluble factors that can dilute target vessel proteins, increase background, and interfere with accurate quantification.
4. Valve and Matrix Barriers
Intrinsic valves and extracellular matrix components such as collagen and elastic fibers can trap proteins and resist solubilization, leading to incomplete extraction.
Advantages
✔️Controlled Lysis of Delicate Vessels
MtoZ Biolabs' Lymphatic Vessel Tissue Protein Extraction Kit uses a carefully balanced lysis buffer system that supports efficient disruption of thin-walled lymphatic vessels while minimizing mechanical damage and sample loss.
✔️Improved Recovery from Low-Input Samples
The extraction chemistry is tuned for low cell density tissues, helping maximize total protein recovery from limited amounts of lymphatic vessel material.
✔️Reduced Lymph and Lipid Interference
The workflow decreases the impact of lymph-derived components and lipids, generating clarified lysates with lower background that are suitable for SDS-PAGE, Western blotting, and LC-MS.
✔️Reliable Performance from MtoZ Biolabs
MtoZ Biolabs formulates the Lymphatic Vessel Tissue Protein Extraction Kit with a straightforward, reproducible protocol, enabling consistent protein yields and data quality across lymphatic vessel samples from different models and experimental designs.
Technical Support
1. Protocol Downloads
MtoZ Biolabs provides comprehensive experimental protocols and product manuals to help ensure smooth and reproducible workflows. For access to specific documents or detailed instructions, please contact our support team.
2. Customized Service Support
Our experienced scientists offer tailored technical assistance and customized solutions based on your specific research goals, helping you achieve optimal experimental outcomes.
3. FAQ and Expert Assistance
We provide responsive and professional technical support to address your questions efficiently. Our experts are ready to help you identify and resolve experimental challenges quickly and accurately.
