MtoZ Biolabs' Heart Tissue Protein Extraction Kit is designed to extract high-quality total protein from dense, contractile heart tissue for downstream biochemical and proteomic analysis.
Product Overview
Extracting total protein from heart tissue with conventional lysis buffers presents multiple challenges. The main issues include:
1. Dense Contractile Fiber Architecture
Cardiac muscle is packed with tightly organized sarcomeres and robust cytoskeletal structures that resist mechanical disruption, often leading to incomplete lysis and low protein recovery when using general-purpose buffers.
2. Collagen-Rich Supporting Matrix
The myocardium contains significant amounts of collagen and other extracellular matrix components that form a tough scaffold, trapping proteins and limiting their efficient solubilization.
3. High Mitochondrial and Enzyme Load
Heart tissue is rich in mitochondria and oxidative enzymes, which can increase background signal, alter buffer conditions, and complicate quantitative analysis if not properly managed during extraction.
4. Interference from Blood and Myoglobin
Residual blood, hemoglobin, and myoglobin introduce additional proteins and pigments that can affect accurate protein quantification and distort electrophoresis and LC-MS readouts.
Advantages
✔️Efficient Lysis of Contractile Myocardium
The Heart Tissue Protein Extraction Kit uses an optimized lysis formulation that penetrates dense cardiac fibers and cytoskeletal structures, supporting thorough cell disruption and robust total protein recovery.
✔️Enhanced Solubilization of Matrix-Associated Proteins
The buffer system helps loosen collagen-rich extracellular matrix and associated components, improving extraction efficiency from both myocardial and perivascular regions.
✔️Managed Mitochondrial and Enzymatic Background
Tailored extraction conditions and a balanced inhibitor system help limit interference from mitochondrial and oxidative enzymes, preserving target proteins and supporting reliable quantitative and functional studies.
✔️Clear, Assay-Ready Lysates from MtoZ Biolabs
MtoZ Biolabs' Heart Tissue Protein Extraction Kit generates clarified lysates with reduced blood and pigment interference, providing samples that are easy to handle and well suited for SDS-PAGE, Western blotting, LC-MS, and related applications.
Technical Support
1. Protocol Downloads
MtoZ Biolabs provides comprehensive experimental protocols and product manuals to help ensure smooth and reproducible workflows. For access to specific documents or detailed instructions, please contact our support team.
2. Customized Service Support
Our experienced scientists offer tailored technical assistance and customized solutions based on your specific research goals, helping you achieve optimal experimental outcomes.
3. FAQ and Expert Assistance
We provide responsive and professional technical support to address your questions efficiently. Our experts are ready to help you identify and resolve experimental challenges quickly and accurately.
