MtoZ Biolabs provides the Islet Tissue Protein Extraction Kit for efficient extraction of high-quality total protein from isolated islet tissue, supporting downstream biochemical and proteomic analysis.
Product Overview
Extracting total protein from small, fragile islet preparations with standard lysis buffers often results in low yield and sample loss. The main reasons include:
1. Limited Starting Material and Low Protein Yield
Pancreatic islets are small and available in limited numbers, so conventional lysis approaches often fail to recover enough protein for parallel assays or quantitative studies.
2. Variable Purity and Exocrine Contamination
Islet preparations frequently contain residual acinar and ductal cells, whose enzymes and proteins can mask islet-specific signals and complicate interpretation of downstream analyses.
3. Fragile Endocrine Cells and Granule-Rich Cytoplasm
β cells and other endocrine cell types are mechanically delicate and packed with secretory granules; harsh homogenization can cause excessive cell rupture, protein degradation, and loss of functional information.
Advantages
✔️High-Efficiency Extraction from Small Islet Samples
MtoZ Biolabs' Islet Tissue Protein Extraction Kit is formulated to maximize protein recovery from limited islet material, enabling robust analysis even when starting with small numbers of islets.
✔️Reduced Impact of Exocrine Contamination
The optimized lysis system supports efficient solubilization of both endocrine and contaminating exocrine components while maintaining a clean, interpretable protein profile for islet-focused studies.
✔️Protection of Labile Endocrine Proteins
A tailored inhibitor cocktail helps preserve proteins and signaling molecules by limiting protease activity during the extraction process.
✔️Low-Viscosity, Assay-Ready Lysates
The workflow minimizes DNA- and granule-induced viscosity, producing clarified lysates that are easy to handle and compatible with SDS-PAGE, Western blotting, LC-MS, and other analytical methods.
Technical Support
1. Protocol Downloads
MtoZ Biolabs provides comprehensive experimental protocols and product manuals to help ensure smooth and reproducible workflows. For access to specific documents or detailed instructions, please contact our support team.
2. Customized Service Support
Our experienced scientists offer tailored technical assistance and customized solutions based on your specific research goals, helping you achieve optimal experimental outcomes.
3. FAQ and Expert Assistance
We provide responsive and professional technical support to address your questions efficiently. Our experts are ready to help you identify and resolve experimental challenges quickly and accurately.
