The Pituitary Gland Tissue Protein Extraction Kit from MtoZ Biolabs is a specialized reagent system for generating high-quality total protein lysates from small, hormone-rich pituitary tissue for downstream biochemical and proteomic studies.
Product Overview
Extracting total proteins from pituitary gland tissue with conventional lysis buffers presents several challenges, including:
1. Heterogeneous Endocrine Cell Populations
The pituitary contains multiple endocrine cell types with distinct granule loads and membrane properties, making uniform lysis difficult and leading to uneven protein recovery.
2. High Hormone and Secretory Granule Content
Dense secretory granules and stored peptide hormones can release large amounts of small proteins and peptides that complicate lysate composition and increase background in downstream assays.
3. Soft, Vascular, and Fragile Tissue
The pituitary is small, highly vascular, and mechanically delicate, so overaggressive homogenization may cause protein degradation, while mild conditions often yield insufficient protein.
4. Viscosity from DNA and Matrix Components
Genomic DNA and extracellular matrix fragments released during disruption increase lysate viscosity, making pipetting difficult and distorting electrophoresis patterns and quantification.
Advantages
✔️Uniform Recovery Across Endocrine Cell Types
MtoZ Biolabs' Pituitary Gland Tissue Protein Extraction Kit is formulated to lyse diverse endocrine cell populations evenly, improving protein recovery from granule-rich and granule-poor cells alike and reducing sample-to-sample variability.
✔️Controlled Disruption of Secretory Granules
Optimized buffer strength and extraction conditions promote efficient granule disruption without overwhelming the lysate with small peptide hormones, helping maintain a balanced protein profile for downstream analysis.
✔️Gentle Yet Efficient Extraction for Fragile Tissue
The kit is tailored for small, soft, and highly vascular pituitary glands, enabling effective homogenization with minimal mechanical stress, which helps limit processing-induced degradation.
✔️Reduced Viscosity and Cleaner Electrophoretic Profiles
Reagents are designed to minimize DNA- and matrix-related viscosity, producing clarified lysates that pipette easily and yield sharper, more interpretable bands in SDS-PAGE and Western blotting, as well as stable signals in LC-MS.
Technical Support
1. Protocol Downloads
MtoZ Biolabs provides comprehensive experimental protocols and product manuals to help ensure smooth and reproducible workflows. For access to specific documents or detailed instructions, please contact our support team.
2. Customized Service Support
Our experienced scientists offer tailored technical assistance and customized solutions based on your specific research goals, helping you achieve optimal experimental outcomes.
3. FAQ and Expert Assistance
We provide responsive and professional technical support to address your questions efficiently. Our experts are ready to help you identify and resolve experimental challenges quickly and accurately.
