The Thymus Tissue Protein Extraction Kit from MtoZ Biolabs is a ready-to-use reagent system for extracting high-quality total protein from lymphocyte-rich thymus tissue for applications such as SDS-PAGE, Western blotting, and LC-MS-based analysis.
Product Overview
Thymus tissue is densely cellular, fragile, and rich in nucleic acids, so routine total protein extraction with general-purpose buffers often leads to poor yield and difficult-to-handle lysates. Key challenges include:
1. Dense Lymphocyte Populations
Thymus is packed with immature T cells, and their tight packing can lead to incomplete lysis or cell clumping when standard extraction conditions are used.
2. Protease- and Nuclease-Rich Environment
Endogenous proteases and nucleases become active upon disruption, degrading target proteins and releasing additional nucleic acids that further compromise sample quality.
3. Delicate Lobular Structure
The soft, lobulated architecture of the thymus is easily damaged by overly harsh mechanical homogenization, which can cause processing-induced degradation if not carefully controlled.
4. High DNA Content and Lysate Viscosity
Rapidly dividing thymocytes release large amounts of genomic DNA during homogenization, creating highly viscous lysates that are hard to pipette and can distort electrophoresis and quantification.
Advantages
✔️Efficient Lysis of Lymphoid Tissue
MtoZ Biolabs' Thymus Tissue Protein Extraction Kit uses an optimized lysis buffer that effectively disrupts densely packed thymocytes and stromal cells, improving total protein recovery from thymic tissue.
✔️Reduced DNA-Induced Viscosity
The extraction workflow is designed to limit DNA-related viscosity and remove excess debris, yielding clarified lysates that are easy to handle and suitable for high-resolution electrophoresis and LC-MS.
✔️Protection of Thymus-Derived Proteins
A tailored inhibitor cocktail suppresses protease and nuclease activity, helping preserve native protein profiles and labile signaling molecules throughout the extraction process.
Technical Support
1. Protocol Downloads
MtoZ Biolabs provides comprehensive experimental protocols and product manuals to help ensure smooth and reproducible workflows. For access to specific documents or detailed instructions, please contact our support team.
2. Customized Service Support
Our experienced scientists offer tailored technical assistance and customized solutions based on your specific research goals, helping you achieve optimal experimental outcomes.
3. FAQ and Expert Assistance
We provide responsive and professional technical support to address your questions efficiently. Our experts are ready to help you identify and resolve experimental challenges quickly and accurately.
