The MtoZ Biolabs Tissue Total Protein Extraction Kit is engineered to efficiently and comprehensively liberate total proteins from diverse animal tissues while effectively overcoming endogenous sources of interference.
Product Overview
During total protein extraction from animal tissues, researchers frequently encounter the following challenges:
1. Incomplete Protein Recovery
Conventional lysis buffers often fail to sufficiently disrupt dense extracellular matrices and membrane structures, resulting in substantial loss of membrane-associated and organelle proteins and leading to poor representativeness of the extracted proteome.
2. Protein Degradation
Endogenous proteases released during tissue lysis can rapidly degrade target proteins if not promptly inhibited.
3. Interference from Viscous Biomolecules
Genomic DNA and lipids liberated upon lysis significantly increase sample viscosity, compromise quantification accuracy, and potentially cause SDS-PAGE loading well blockage, elevated Western blot background, or distorted band morphology.
Advantages
1. Deep Tissue Disruption with High Protein Yield
The MtoZ Biolabs Tissue Total Protein Extraction Kit utilizes an optimized combination of potent detergents and reducing agents to efficiently disrupt tissue organization and all types of biological membranes, ensuring robust release of cytoplasmic, nuclear, membrane, and organellar proteins for comprehensive proteome acquisition.
2. Protection of Protein Integrity Through Rapid Inhibition of Proteolysis
A highly effective broad-spectrum protease inhibitor cocktail is pre-included in the formulation, enabling immediate suppression of protease activity upon lysis and preservation of native protein structure and integrity.
3. Minimized Interference and Superior Compatibility with Downstream Applications
The unique lysis chemistry substantially reduces DNA- and lipid-induced viscosity, yielding highly pure, low-viscosity protein samples that are directly amenable to quantitative analysis, Western blotting, immunoprecipitation, and mass spectrometry without the need for additional purification steps.
Technical Support
1. Protocol Downloads
MtoZ Biolabs provides comprehensive experimental protocols and product manuals to help ensure smooth and reproducible workflows. For access to specific documents or detailed instructions, please contact our support team.
2. Customized Service Support
Our experienced scientists offer tailored technical assistance and customized solutions based on your specific research goals, helping you achieve optimal experimental outcomes.
3. FAQ and Expert Assistance
We provide responsive and professional technical support to address your questions efficiently. Our experts are ready to help you identify and resolve experimental challenges quickly and accurately.
