The Bone Marrow Tissue Protein Extraction Kit from MtoZ Biolabs is built to obtain clean, high-quality total protein from bone marrow samples while minimizing interference from blood rich components, lipids, and highly active endogenous enzymes.
Product Overview
Bone marrow is a complex, heterogeneous matrix that can be difficult to lyse consistently without compromising protein integrity. The main challenges of its protein extraction include:
1. Mixed Cell and Matrix Composition
Bone marrow combines suspended hematopoietic cells with stromal cells and extracellular matrix, making it difficult to achieve uniform disruption and reproducible protein recovery using standard cell or tissue lysis protocols.
2. High DNA Content and Clotting Tendency
Rupture of large numbers of nucleated cells releases abundant genomic DNA and can promote clot-like aggregates, generating highly viscous lysates that are hard to pipette and that interfere with accurate separation and quantification.
3. Lipid and Debris from Fatty Marrow
Adipocytes and marrow fat contribute lipids and particulates that cloud lysates, complicate downstream electrophoresis and chromatography, and may mask low-abundance proteins.
4. Enzyme-Rich Microenvironment
Endogenous proteases and nucleases activated during processing can degrade target proteins and release additional nucleic acids, compromising sample integrity if not controlled promptly.
Advantages
✔️Targeted for Heterogeneous Marrow Samples
MtoZ Biolabs' Bone Marrow Tissue Protein Extraction Kit employs a reagent system tailored to lyse both hematopoietic and stromal components efficiently, supporting uniform cell disruption and robust total protein recovery from aspirates or core-derived marrow.
✔️Protection of Labile Marrow Proteins
A dedicated inhibitor mixture helps suppress protease and nuclease activity during processing, preserving native protein profiles, including signaling and differentiation markers that are often sensitive to degradation.
✔️Lower Viscosity and Cleaner Lysates
The extraction workflow is designed to limit DNA-driven gel formation and reduce particulate carryover, yielding clarified, low-viscosity lysates that are easier to handle and suitable for high-resolution SDS-PAGE, Western blotting, and LC-MS analysis.
Technical Support
1. Protocol Downloads
MtoZ Biolabs provides comprehensive experimental protocols and product manuals to help ensure smooth and reproducible workflows. For access to specific documents or detailed instructions, please contact our support team.
2. Customized Service Support
Our experienced scientists offer tailored technical assistance and customized solutions based on your specific research goals, helping you achieve optimal experimental outcomes.
3. FAQ and Expert Assistance
We provide responsive and professional technical support to address your questions efficiently. Our experts are ready to help you identify and resolve experimental challenges quickly and accurately.
