MtoZ Biolabs' Nuclear Membrane and Nuclear Protein Extraction Kit is designed to extract high-quality total nuclear proteins together with nuclear membrane proteins from cultured cells and tissue samples for downstream Western blotting, LC-MS-based proteomics, and transcription factor analysis.
Product Overview
Conventional whole cell lysis often fails to efficiently enrich nuclear membranes and nuclear proteins while maintaining purity and functionality. Key challenges include:
1. Incomplete Nuclear Enrichment
Standard lysis conditions may not fully separate nuclei from cytoplasmic components, leading to contamination of nuclear fractions with abundant cytosolic proteins.
2. Tight Association with Chromatin
Many nuclear proteins, including transcription factors and regulatory complexes, are tightly bound to DNA and chromatin, making them difficult to solubilize without harsh conditions that compromise function.
3. Recovery of Nuclear Membrane Proteins
Integral and peripheral nuclear envelope proteins are embedded in lipid bilayers, so they are often underrepresented or lost when using generic membrane or nuclear extraction buffers.
4. Viscosity from Genomic DNA
Disruption of nuclei releases large amounts of genomic DNA that can rapidly increase viscosity, complicating pipetting and affecting the reliability of electrophoresis and quantitative analysis.
Advantages
✔️Comprehensive Extraction of Nuclear Proteins
The Nuclear Membrane and Nuclear Protein Extraction Kit provides a stepwise lysis strategy that enriches nuclei and then releases both total nuclear proteins and nuclear membrane proteins, supporting broad coverage of the nuclear proteome from a single sample.
✔️Efficient Release of Chromatin-Associated Proteins
An optimized extraction system helps liberate DNA- and chromatin-bound proteins under controlled conditions, improving recovery of transcription factors and regulatory proteins while maintaining their detectability in downstream assays.
✔️Improved Yield of Nuclear Membrane Proteins
The buffer composition is tuned to solubilize nuclear envelope proteins and associated complexes, enhancing detection of low-abundance nuclear membrane targets that are often missed with standard lysis reagents.
✔️Controlled DNA Handling and Reduced Viscosity
The workflow incorporates conditions that limit DNA-induced viscosity and facilitate removal or reduction of nucleic acid interference, yielding clarified nuclear extracts that are easier to handle and produce sharper bands and more reliable quantitative data.
Technical Support
1. Protocol Downloads
MtoZ Biolabs provides comprehensive experimental protocols and product manuals to help ensure smooth and reproducible workflows. For access to specific documents or detailed instructions, please contact our support team.
2. Customized Service Support
Our experienced scientists offer tailored technical assistance and customized solutions based on your specific research goals, helping you achieve optimal experimental outcomes.
3. FAQ and Expert Assistance
We provide responsive and professional technical support to address your questions efficiently. Our experts are ready to help you identify and resolve experimental challenges quickly and accurately.
