MtoZ Biolabs' insoluble protein extraction kit (tissue) is specifically designed for poorly soluble tissue proteins. It can efficiently dissolve and separate refractory proteins, supporting the analysis and research of insoluble proteins in complex tissues.
Product Overview
Proteins in insoluble tissues are typically highly hydrophobic, tightly folded, and strongly associated with lipids, the cytoskeleton, or membrane structures. These characteristics pose unique challenges for protein extraction:
1. Difficulty in Protein Solubilization
Insoluble proteins easily aggregate and precipitate, and conventional buffers cannot dissolve them effectively, seriously affecting protein recovery.
2. Tight Association with Membranes or the Cytoskeleton
Many poorly soluble proteins are embedded in membrane structures or interact with cytoskeletal proteins, making them difficult to release through traditional lysis methods.
3. Precipitates Are Difficult to Re-Dissolve
Protein precipitates formed during tissue processing exhibit stable structures, making them difficult to re-dissolve even after repeated extraction attempts.
4. Extensive Impurity Interference
Lipids, membrane fragments, and highly polymerized substances readily co-precipitate with insoluble proteins, reducing protein purity.
Advantages
✅ Strong Solubilization System
The specialized buffer system can significantly improve the solubility of insoluble proteins and effectively disrupt hydrophobic aggregation.
✅ Efficient Membrane and Structural Dissociation Capability
Optimized lysis components can break the interactions between proteins and membrane structures or the cytoskeleton, enhancing protein release efficiency.
✅ Enhanced Re-Solubilization Performance
MtoZ Biolabs' insoluble protein extraction kit (tissue) provides excellent re-solubilization capability for refractory precipitates, ensuring sufficient recovery of poorly soluble proteins.
✅ Removal of Lipids and Impurities
Built-in clarification and impurity-removal modules reduce membrane-related contaminants and lipid interference, yielding higher-purity proteins.
✅ Compatibility with Multiple Analytical Platforms
The extracted proteins can be directly used for SDS-PAGE, Western blot, LC-MS/MS, and other downstream analyses, improving experimental reliability.
Technical Support
1. Protocol Downloads
MtoZ Biolabs provides comprehensive experimental protocols and product manuals to help ensure smooth and reproducible workflows. For access to specific documents or detailed instructions, please contact our support team.
2. Customized Service Support
Our experienced scientists offer tailored technical assistance and customized solutions based on your specific research goals, helping you achieve optimal experimental outcomes.
3. FAQ and Expert Assistance
We provide responsive and professional technical support to address your questions efficiently. Our experts are ready to help you identify and resolve experimental challenges quickly and accurately.
