The Yeast Histone Extraction Kit from MtoZ Biolabs is specifically designed for yeast chromatin's dense structure, overcoming challenges such as difficult histone release and impurity interference, and supporting epigenetics and chromatin regulation research.
Product Overview
Yeast chromatin has a compact structure, low histone content, and a complex metabolic background, presenting unique challenges for histone extraction:
1. Highly Dense Chromatin, Difficult Histone Release
Yeast histones are tightly packed within the dense nuclear structure, and conventional disruption methods are insufficient to fully release histones, leading to low recovery rates.
2. Tough Cell Wall, Difficulty in Releasing Nuclear Components
Yeast cell walls are composed of β-glucan and chitin, which are structurally stable, making it difficult to efficiently break the cell wall and release nuclear components, thus increasing histone extraction difficulty.
3. High Contamination from Nucleic Acids and Polysaccharides
Yeast contains abundant nucleic acids and polysaccharides, which co-extract with histones, affecting sample purity and interfering with downstream analyses such as mass spectrometry or ChIP.
4. Risk of Histone Modification Damage
Traditional acid extraction methods are harsh and time-consuming, which may result in the loss or alteration of critical epigenetic marks such as acetylation and methylation.
Advantages
✅ Efficient
Optimized enzymatic and auxiliary lysis methods effectively break the yeast cell wall and release nuclear histones, improving recovery rates.
✅ Dedicated
The selective extraction formulation in MtoZ Biolabs' Yeast Histone Extraction Kit is specifically designed for low-abundance histones, significantly reducing background protein interference and ensuring the extraction of high-purity samples.
✅ Gentle
Non-denaturing conditions combined with modification protectants maximize the retention of natural histone modification patterns that reflect the biological state.
✅ Compatible
The extracted histones are directly compatible with ChIP, Western blot, mass spectrometry, and other epigenetic experiments.
Technical Support
1. Protocol Downloads
MtoZ Biolabs provides comprehensive experimental protocols and product manuals to help ensure smooth and reproducible workflows. For access to specific documents or detailed instructions, please contact our support team.
2. Customized Service Support
Our experienced scientists offer tailored technical assistance and customized solutions based on your specific research goals, helping you achieve optimal experimental outcomes.
3. FAQ and Expert Assistance
We provide responsive and professional technical support to address your questions efficiently. Our experts are ready to help you identify and resolve experimental challenges quickly and accurately.
