MtoZ Biolabs' Metaproteomic Protein Extraction Kit (Microbial Pellet Samples) is a dedicated reagent system for extracting high-quality microbial proteins from mixed community pellets for LC-MS-based metaproteomics and functional microbiology studies.
Product Overview
Microbial pellets collected from complex communities are more difficult to process than single strain cultures, and standard cell lysis buffers often fail to recover proteins evenly across taxa. The main challenges include
1. Diverse Cell Envelope Structures
Community pellets can contain Gram-positive and Gram-negative bacteria, spores, fungi, and archaea, so a single mild lysis buffer often leaves robust cell types underlysed and underrepresented in the final protein extract.
2. Extracellular Polymeric Substances and Matrix
Biofilm like extracellular polymeric substances and secreted polysaccharides trap cells and proteins, increase viscosity, and make it harder to obtain clear, homogeneous lysates suitable for downstream analysis.
3. Dense, Compact Pellets
High cell density and tight pellet packing lead to incomplete resuspension and uneven exposure to lysis reagents, which causes variable protein yields and poor reproducibility between samples.
4. Medium Components and Process Additives
Residual media, salts, detergents, and other process additives carried into the pellet can co-extract with proteins and interfere with SDS-PAGE separation and LC-MS ionization efficiency.
Advantages
✔️Broad Lysis of Mixed Microbial Communities
The Metaproteomic Protein Extraction Kit (Microbial Pellet Samples) uses a lysis formulation and workflow tuned to disrupt a wide range of microbial cell envelopes, improving protein recovery from both easily lysed and more resilient community members.
✔️Less EPS and Matrix Interference
Extraction and clarification steps are designed to reduce extracellular polymeric substances (EPS) and polysaccharide carryover, lowering viscosity and producing clearer lysates that are easier to handle and more compatible with electrophoresis and LC-MS.
✔️Efficient Processing of Dense Pellets
The protocol promotes effective resuspension and penetration of lysis buffers into compact microbial pellets, helping achieve more uniform extraction and more consistent protein yields across replicates.
✔️Cleaner, Assay Ready Extracts
MtoZ Biolabs' Metaproteomic Protein Extraction Kit (Microbial Pellet Samples) helps minimize interference from residual media components, generating protein extracts that load predictably on gels and deliver more reliable metaproteomic data.
Technical Support
1. Protocol Downloads
MtoZ Biolabs provides comprehensive experimental protocols and product manuals to help ensure smooth and reproducible workflows. For access to specific documents or detailed instructions, please contact our support team.
2. Customized Service Support
Our experienced scientists offer tailored technical assistance and customized solutions based on your specific research goals, helping you achieve optimal experimental outcomes.
3. FAQ and Expert Assistance
We provide responsive and professional technical support to address your questions efficiently. Our experts are ready to help you identify and resolve experimental challenges quickly and accurately.
