MtoZ Biolabs' Yeast Endoplasmic Reticulum Membrane Protein Extraction Kit is specifically designed for the isolation of yeast endoplasmic reticulum membrane proteins, featuring efficient cell wall disruption and removal of impurities. The kit increases membrane protein yield and supports studies of membrane protein function and interactions.
Product Overview
Yeast endoplasmic reticulum membrane proteins are highly hydrophobic and deeply embedded in the ER structure, with poor stability and solubility, which poses specific challenges for protein extraction:
1. Dense Cell Wall and Difficult Disruption
The rigid yeast cell wall makes conventional lysis methods inefficient, resulting in insufficient release of membrane proteins.
2. Complex Endoplasmic Reticulum Architecture
Membrane proteins are embedded in the lipid bilayer and are prone to incomplete extraction or structural disruption during isolation.
3. High Lipid and Debris Interference
After lysis, abundant liposomes and organelle fragments interfere with membrane protein purification and compromise the accuracy of downstream analyses.
4. Strong Hydrophobicity and Aggregation of Membrane Proteins
Traditional lysis buffers often fail to maintain solubility, leading to precipitation and protein loss.
Advantages
✅ Robust
Optimized enzymatic digestion combined with mechanical disruption increases the release efficiency of endoplasmic reticulum membrane proteins.
✅ Optimized
Carefully controlled detergent type and concentration stabilize hydrophobic membrane proteins and reduce aggregation and precipitation.
✅ Efficient
MtoZ Biolabs' Yeast Endoplasmic Reticulum Membrane Protein Extraction Kit efficiently removes lipid impurities and cellular debris to obtain membrane protein samples with a cleaner background.
✅ Compatible
The extracted membrane proteins are suitable for Western blot, LC-MS/MS, affinity-based enrichment, and functional analysis.
Technical Support
1. Protocol Downloads
MtoZ Biolabs provides comprehensive experimental protocols and product manuals to help ensure smooth and reproducible workflows. For access to specific documents or detailed instructions, please contact our support team.
2. Customized Service Support
Our experienced scientists offer tailored technical assistance and customized solutions based on your specific research goals, helping you achieve optimal experimental outcomes.
3. FAQ and Expert Assistance
We provide responsive and professional technical support to address your questions efficiently. Our experts are ready to help you identify and resolve experimental challenges quickly and accurately.
