The Yeast Golgi Apparatus Protein Extraction Kit launched by MtoZ Biolabs is specially designed for yeast with complex organelle structures. It can directionally isolate Golgi apparatus proteins and is suitable for analysis of proteins related to the secretion pathway.
Product Overview
Yeast cells are characterized by an intact cell wall structure, a highly developed intracellular membrane system, and a highly dynamic Golgi apparatus. These complex subcellular features pose specific challenges for the accurate isolation and enrichment of Golgi apparatus proteins:
1. Cell Wall Interference with Isolation
The yeast cell wall is composed of glucans and mannans and exhibits high mechanical strength. Insufficient cell disruption can affect the intact release of organelles in subsequent steps.
2. Highly Complex Membrane System
The Golgi apparatus is closely connected to the endoplasmic reticulum and transport vesicles. Improper lysis can easily lead to mixing of membrane systems, reducing the specificity of Golgi protein isolation.
3. Fragile Golgi Structure
The stacked membrane architecture of the Golgi apparatus is sensitive and can be disrupted during isolation, resulting in the loss of associated proteins or mislocalized distribution.
4. Cytoplasmic Protein Interference
Large amounts of soluble cytoplasmic proteins can readily co-exist with Golgi fractions during lysis, affecting sample purity and the accuracy of downstream analyses.
Advantages
✅ Mild Cell Disruption Strategy
The lysis conditions are optimized according to the characteristics of the yeast cell wall, enabling efficient cell disruption while minimizing nonspecific damage to membrane-bound organelles.
✅ Targeted Organelle Separation
The stepwise fractionation workflow helps distinguish the Golgi apparatus from other membrane-bound organelles, improving targeted enrichment efficiency.
✅ Reduced Background Interference
Optimized separation parameters effectively reduce the carryover of cytoplasmic proteins and non-target membrane components, yielding cleaner Golgi protein samples.
✅ Reduced Cytoplasmic Protein Background
The clarification and cleanup steps integrated into the MtoZ Biolabs' Yeast Golgi apparatus protein extraction kit effectively decrease residual soluble cytoplasmic proteins, resulting in a clearer sample background.
✅ Compatibility with Downstream Analysis
The extracted Golgi apparatus proteins can be directly applied to LC-MS/MS, SDS-PAGE, Western blot, and related platforms, ensuring stable data quality and good reproducibility.
Technical Support
1. Protocol Downloads
MtoZ Biolabs provides comprehensive experimental protocols and product manuals to help ensure smooth and reproducible workflows. For access to specific documents or detailed instructions, please contact our support team.
2. Customized Service Support
Our experienced scientists offer tailored technical assistance and customized solutions based on your specific research goals, helping you achieve optimal experimental outcomes.
3. FAQ and Expert Assistance
We provide responsive and professional technical support to address your questions efficiently. Our experts are ready to help you identify and resolve experimental challenges quickly and accurately.
