MtoZ Biolabs' Algae Insoluble Protein Extraction Kit is formulated to recover insoluble proteins from algal cells and biomass for downstream SDS-PAGE, Western blotting, and mass spectrometry-based analysis.
Product Overview
Isolating insoluble proteins from algae with standard lysis buffers is complicated. The main challenges include:
1. Rigid Cell Walls and Tough Biomass
Many algae possess thick polysaccharide rich walls and dense biomass that resist conventional lysis, leading to incomplete cell disruption and low protein recovery.
2. Pigments and Polysaccharides Interference
Chlorophyll, other pigments, and extracellular polysaccharides co-extract with proteins, increasing viscosity, raising background, and affecting SDS-PAGE and LC-MS performance.
3. Strongly Bound Membrane and Thylakoid Proteins
Insoluble and membrane-associated proteins, including thylakoid components, often remain trapped in pellets or partially solubilized when only mild detergents or simple buffers are used.
4. Aggregation of Insoluble Protein Fractions
Insoluble proteins tend to form aggregates that are difficult to resuspend uniformly, which complicates quantification and reduces reproducibility in comparative studies.
Advantages
✔️Efficient Disruption of Algal Cells
The Algae Insoluble Protein Extraction Kit from MtoZ Biolabs supports effective lysis of tough algal biomass, improving release of insoluble proteins compared with generic cell lysis reagents.
✔️Lower Pigment and Polysaccharide Carryover
The extraction workflow is designed to limit chlorophyll and polysaccharide contamination, yielding cleaner protein preparations that run more predictably in SDS-PAGE and perform better in LC-MS.
✔️Improved Solubilization of Insoluble and Membrane Proteins
Optimized buffer components enhance the solubilization of pellet-associated and membrane bound proteins, increasing the representation of target insoluble proteins in the final extract.
✔️Reproducible, Assay Ready Fractions
MtoZ Biolabs formulates the Algae Insoluble Protein Extraction Kit with a straightforward, repeatable protocol, providing dispersible insoluble protein fractions that are easier to quantify and compare across experiments.
Technical Support
1. Protocol Downloads
MtoZ Biolabs provides comprehensive experimental protocols and product manuals to help ensure smooth and reproducible workflows. For access to specific documents or detailed instructions, please contact our support team.
2. Customized Service Support
Our experienced scientists offer tailored technical assistance and customized solutions based on your specific research goals, helping you achieve optimal experimental outcomes.
3. FAQ and Expert Assistance
We provide responsive and professional technical support to address your questions efficiently. Our experts are ready to help you identify and resolve experimental challenges quickly and accurately.
