MtoZ Biolabs' Filamentous Fungal Endoplasmic Reticulum Protein Extraction Kit is designed to efficiently enrich and extract ER associated proteins from filamentous fungi while minimizing losses caused by rigid cell walls, abundant polysaccharides, and membrane rich organelles.
Product Overview
Endoplasmic reticulum protein extraction from filamentous fungi is technically demanding. The main challenges include:
1. Tough Cell Wall and Hyphal Network
Filamentous fungi possess thick chitin and glucan rich cell walls and intertwined hyphae that resist mechanical disruption, so standard lysis buffers often fail to efficiently break cells and expose intracellular organelles.
2. Low Abundance of ER Proteins in Whole Lysates
ER proteins represent only a small fraction of total cellular protein and are easily overwhelmed by cytosolic and plasma membrane proteins when simple whole cell lysis is used.
3. Membrane Complexity and Organelle Cross Contamination
ER membranes are closely associated with Golgi, plasma membrane, and mitochondria; non-selective extraction frequently produces mixed membrane fractions that reduce the specificity of ER protein studies.
4. Protease and Glycosidase Activity in Secretory Cells
Secretory and enzyme producing fungi express high levels of proteases and glycosidases that can degrade ER resident proteins or alter their glycosylation state during sample processing.
Advantages
✔️ER Oriented Enrichment Strategy
MtoZ Biolabs' Filamentous Fungal Endoplasmic Reticulum Protein Extraction Kit applies a stepwise extraction workflow that combines optimized cell disruption with ER biased enrichment, improving the representation of ER resident proteins in the final lysate.
✔️Improved Lysis of Filamentous Fungi
Reagents and conditions are tuned for tough, hypha forming fungi, promoting effective wall weakening and intracellular release while limiting excessive fragmentation that can complicate downstream clarification.
✔️Reduced Organelle and Plasma Membrane Carryover
The buffer system supports differential solubilization of membranes, helping to reduce contamination from mitochondria and plasma membrane and increasing the specificity of ER focused Western blotting and LC-MS experiments.
✔️Protection of ER Associated Proteins
A tailored inhibitor mix helps control protease and glycosidase activity, preserving the integrity of ER resident proteins and their post translational modifications during extraction.
Technical Support
1. Protocol Downloads
MtoZ Biolabs provides comprehensive experimental protocols and product manuals to help ensure smooth and reproducible workflows. For access to specific documents or detailed instructions, please contact our support team.
2. Customized Service Support
Our experienced scientists offer tailored technical assistance and customized solutions based on your specific research goals, helping you achieve optimal experimental outcomes.
3. FAQ and Expert Assistance
We provide responsive and professional technical support to address your questions efficiently. Our experts are ready to help you identify and resolve experimental challenges quickly and accurately.
