MtoZ Biolabs provides the Bacterial Outer Membrane Protein Extraction Kit for selective isolation of outer membrane proteins from Gram-negative bacteria, supporting downstream protein studies such as SDS-PAGE, Western blotting, and LC-MS-based proteomic analysis.
Product Overview
Outer membrane proteins from Gram-negative bacteria are hard to isolate with standard lysis buffers. The main technical challenges include:
1. Complex Gram-Negative Cell Envelope
The outer membrane, peptidoglycan layer, and inner membrane form a tightly integrated barrier that is hard to disrupt in a controlled way, often leading to incomplete lysis or non-selective release of proteins.
2. Poor Separation of Outer and Inner Membranes
Conventional lysis procedures tend to co-isolate inner membrane and cytoplasmic proteins with outer membrane proteins, reducing the specificity of outer membrane studies and complicating data interpretation.
3. Lipid and LPS Related Interference
High levels of lipopolysaccharides and membrane lipids in the outer membrane can cause aggregation, affect protein solubility, and interfere with electrophoresis, chromatography, and mass spectrometry.
4. Risk of Denaturing Surface Exposed Proteins
Harsh detergents or excessive sonication can disrupt native outer membrane protein complexes, mask epitopes, and alter conformations that are critical for functional assays or antibody-based detection.
Advantages
✔️Selective Enrichment of Outer Membrane Proteins
MtoZ Biolabs' Bacterial Outer Membrane Protein Extraction Kit employs a reagent system and workflow tuned to favor outer membrane recovery while reducing inner membrane and cytosolic carryover, providing more specific outer membrane protein fractions.
✔️Controlled Cell Disruption and Envelope Fractionation
Optimized extraction conditions promote efficient breakage of the bacterial envelope while maintaining the integrity of outer membrane proteins and limiting over disruption that leads to mixed membrane contamination.
✔️Improved Handling of LPS and Lipids
The buffer system is formulated to manage lipopolysaccharides and other membrane lipids, reducing aggregation and producing outer membrane protein samples that are more compatible with SDS-PAGE, Western blotting, and LC-MS analysis.
✔️Mild Extraction to Reduce Denaturation Risk
Conditions of the kit are designed to reduce over-solubilization and, where possible, help retain outer membrane protein structure and complex integrity, supporting functional studies and antibody-based detection.
Technical Support
1. Protocol Downloads
MtoZ Biolabs provides comprehensive experimental protocols and product manuals to help ensure smooth and reproducible workflows. For access to specific documents or detailed instructions, please contact our support team.
2. Customized Service Support
Our experienced scientists offer tailored technical assistance and customized solutions based on your specific research goals, helping you achieve optimal experimental outcomes.
3. FAQ and Expert Assistance
We provide responsive and professional technical support to address your questions efficiently. Our experts are ready to help you identify and resolve experimental challenges quickly and accurately.
