MtoZ Biolabs' Metaproteomic Protein Extraction Kit (Soil Samples) is designed to extract microbial proteins from complex soil matrices for downstream LC-MS-based metaproteomics, functional microbiome studies, and environmental protein profiling.
Product Overview
Recovering proteins for metaproteomics from soil is difficult. The main challenges include:
1. Heterogeneous Soil Composition
Soil contains a mixture of minerals, organic matter, microbes, plant residues, and colloids, making it hard to apply a single lysis strategy that efficiently disrupts diverse microbial cells while keeping extracts comparable between samples.
2. Humic Substances and Organic Contaminants
Humic and fulvic acids, phenolics, and other organic compounds co-extract with proteins, darken lysates, suppress ionization in LC-MS, and increase background in gel-based analysis.
3. Protein Adsorption to Soil Particles
Clay minerals, metal oxides, and organic coated particles can bind proteins and enzymes strongly, leading to low apparent yield and biased recovery of certain taxa or functional groups.
4. Low and Uneven Microbial Biomass
In many soils, microbial biomass is relatively low or patchy, so standard extraction protocols often do not recover enough protein for replicate LC-MS runs or quantitative metaproteomic comparisons.
Advantages
✔️Extraction Chemistry Tailored for Soil Matrices
The Metaproteomic Protein Extraction Kit (Soil Samples) applies a reagent system optimized for complex soil, improving lysis across diverse microbial communities and supporting more consistent protein recovery between different soil types.
✔️Reduced Impact of Humic and Organic Interference
The kit includes buffer components and clarification steps that help separate humic substances and other organic contaminants from the protein fraction, producing lighter, cleaner extracts that perform better in SDS-PAGE and LC-MS.
✔️Improved Release of Particle Bound Proteins
Formulation and processing conditions are designed to disrupt protein–particle interactions, enhancing desorption of proteins from clay and mineral surfaces and increasing the detectable fraction of soil microbial proteins.
✔️Enhanced Yield from Low Biomass Soils
MtoZ Biolabs' Metaproteomic Protein Extraction Kit (Soil Samples) combines efficient microbial cell lysis with a low volume, low loss workflow to increase protein recovery from small or low biomass soil samples, providing sufficient material for replicate metaproteomic analysis.
Technical Support
1. Protocol Downloads
MtoZ Biolabs provides comprehensive experimental protocols and product manuals to help ensure smooth and reproducible workflows. For access to specific documents or detailed instructions, please contact our support team.
2. Customized Service Support
Our experienced scientists offer tailored technical assistance and customized solutions based on your specific research goals, helping you achieve optimal experimental outcomes.
3. FAQ and Expert Assistance
We provide responsive and professional technical support to address your questions efficiently. Our experts are ready to help you identify and resolve experimental challenges quickly and accurately.
