The gram-negative bacterial membrane protein extraction kit launched by MtoZ Biolabs is specially designed for gram-negative bacteria with a double-layer membrane structure. It can effectively distinguish between inner and outer membrane proteins and is suitable for fine analysis of membrane proteins.
Product Overview
Gram-negative bacteria exhibit typical characteristics such as an inner and outer double-membrane structure, an outer membrane rich in lipopolysaccharides, a well-defined periplasmic space, and complex membrane protein distribution. These structural attributes pose multiple challenges for the isolation and enrichment of membrane proteins:
1. Complex Double-Membrane Structure
Gram-negative bacteria possess both inner and outer membranes, with distinct protein compositions across different membrane layers. Conventional lysis methods struggle to achieve effective separation without compromising structural integrity.
2. Significant Lipopolysaccharide Interference
Large amounts of lipopolysaccharides in the outer membrane readily coexist with membrane proteins during extraction, affecting solubility, clarification, and the stability of downstream analyses.
3. Diverse Membrane Protein Types
Porins, transporters, and receptor proteins are distributed across different membrane layers. Inappropriate extraction conditions can lead to insufficient enrichment or bias toward specific classes of membrane proteins.
4. Periplasmic Protein Contamination
During cell disruption, proteins from the periplasmic space can easily enter membrane fractions, reducing the specificity and purity of membrane protein samples.
Advantages
✅ Adapted for Double-Membrane Structure
The lysis and separation system is optimized for the double-membrane characteristics of gram-negative bacteria, helping to more appropriately handle proteins originating from different membrane layers during extraction.
✅ Reduced Lipopolysaccharide Residues
The accompanying processing workflow effectively reduces residual lipopolysaccharides from the outer membrane, improving membrane protein solubility and sample purity.
✅ Improved Extraction Consistency
The extraction conditions of the gram-negative bacterial membrane protein extraction kit from MtoZ Biolabs accommodate different types of membrane proteins, reducing extraction bias.
✅ Reduced Periplasmic Contamination
The separation steps help lower the proportion of periplasmic protein carryover, resulting in clearer and more reliable membrane protein fractions.
✅ Compatibility with Major Analytical Platforms
The extracted membrane proteins can be directly applied to LC-MS/MS, SDS-PAGE, Western blot, and other analytical platforms, ensuring stable and reproducible downstream data.
Technical Support
1. Protocol Downloads
MtoZ Biolabs provides comprehensive experimental protocols and product manuals to help ensure smooth and reproducible workflows. For access to specific documents or detailed instructions, please contact our support team.
2. Customized Service Support
Our experienced scientists offer tailored technical assistance and customized solutions based on your specific research goals, helping you achieve optimal experimental outcomes.
3. FAQ and Expert Assistance
We provide responsive and professional technical support to address your questions efficiently. Our experts are ready to help you identify and resolve experimental challenges quickly and accurately.
