Fungus (Yeast) Protein Extraction Kit

Product Name	Fungal (Yeast) Protein Extraction Kit	Storage Conditions	4°C
Kit Components	Extraction Buffer, PMSF	Applicable Organisms	Unicellular fungal samples
Product Type	Kit	Shelf Life	1 year
Product Size	48T/box	Compatible Applications	Protein identification, proteomics, IP, WB

 

Protein is a core molecule of life activities and is extensively involved in biological processes such as cellular metabolism, signal transduction, and gene regulation. Studying the structure and function of proteins is of great significance for understanding cell physiology, biosynthesis, disease mechanisms, and drug development. In biological research and industrial applications, yeast and other fungi are widely used as important model organisms, expression systems, and fermentation strains for functional protein production, metabolic research, genetic engineering, and other fields.

 

Due to the tough and complex structure of the yeast-like fungal cell wall, traditional protein extraction methods are often inefficient, cumbersome to operate, and may lead to protein denaturation or loss of activity. Therefore, optimizing protein extraction methods and improving protein yield and quality are critical to the success of experiments. The fungus (yeast) protein extraction kit adopts a mild and efficient chemical lysis system specifically designed for yeast-like fungi. It can rapidly and efficiently lyse cells to release intracellular proteins, while avoiding protein denaturation caused by mechanical methods, providing a more stable and reliable protein extraction solution.

 

Product Overview

The fungus (yeast) protein extraction kit from MtoZ Biolabs is specially designed for yeast-like fungi and uses an optimized chemical lysis buffer system that efficiently disrupts the cell wall and rapidly releases intracellular proteins. The kit is compatible with a variety of downstream applications such as SDS-PAGE, Western blotting, and LC-MS/MS proteomics analysis, and is suitable for use in scientific research, industrial studies, and biopharmaceutical development.

 

Protocol

The fungus (yeast) protein extraction kit adopts an optimized chemical lysis system that enables efficient extraction of proteins from yeast-like fungi in a short period of time, significantly improving experimental efficiency and adapting to various laboratory environments and experimental scales. The following are the recommended procedures for use, which researchers may adjust according to their specific needs.

1. Fungal Cell Culture and Collection

(1) According to experimental requirements, culture the fungi to the logarithmic growth phase to ensure good cell growth status.

(2) Centrifuge at 4°C, 5000 ×g for 10 minutes to remove the culture medium and collect the fungal cell pellet.

 

2. Cell Lysis

(1) Add fungal protein extraction buffer (provided in the fungus (yeast) protein extraction kit) at the recommended ratio and fully resuspend the cell pellet.

Note: It is recommended to add 200–300 μL of extraction buffer per 50 μL of fungal pellet.

(2) Pipette the cell suspension up and down to mix thoroughly, then vortex for 10 minutes to promote cell wall disruption.

(3) Sonicate for 10 minutes (60% power, 3 seconds on, 3 seconds off) to ensure complete lysis of fungal cells.

 

3. Centrifugation to Remove Cell Debris

(1) Centrifuge the lysate at 4°C, 12000 ×g for 10 minutes to remove incompletely lysed cell fragments and remaining cell wall/membrane components.

(2) Collect the supernatant, which contains the extracted proteins, and proceed to downstream applications directly.

 

4. Protein Storage

(1) For short-term storage (1-2 weeks), keep the protein extract at 4°C.

(2) For long-term storage (>2 weeks), it is recommended to add 20% glycerol and store at -80°C to prevent protein degradation and reduce the impact of repeated freeze-thaw cycles on protein activity.

The entire procedure can be completed within 30 minutes without the need for additional advanced equipment, improving experimental efficiency and minimizing sample loss.

 

Figures

 

Figure 1. Extraction Effect Diagram of Fungus (Yeast) Protein Extraction Kit.

  

Product Notes

1. Avoid prolonged high-temperature incubation to prevent protein degradation.

2. To minimize degradation during storage, avoid repeated freeze-thaw cycles; aliquoting the protein extract is recommended.

3. The volume of lysis buffer can be adjusted based on experimental needs to optimize protein extraction efficiency.

  

Features and Benefits

1. Efficient Lysis

Utilizes an optimized chemical buffer system to minimize protein denaturation and degradation.

 

2. High Reproducibility and Data Consistency

The standardized, optimized lysis protocol ensures consistency and stability across different experimental batches.

 

3. Compatible with Various Downstream Applications

Fungal protein extracts yield high protein content, suitable for SDS-PAGE, Western Blot, ELISA, LC-MS/MS, and other assays.

 

4. Simple Operation

Ready-to-use reagents eliminate the need for complex optimization, enabling protein extraction to be completed within 30 minutes and significantly reducing experiment time.

 

Applications

1. Proteomics Research

Extract high-quality proteins for LC-MS/MS analysis to profile protein expression in yeast-like fungi and investigate their physiological metabolism and environmental adaptability.

 

2. Western Blot & ELISA

Obtain high-quality protein samples for qualitative and quantitative detection of target proteins in yeast-like fungi.

 

3. Recombinant Protein Expression Analysis

Suitable for protein extraction from yeast hosts to evaluate the effects of different expression conditions on recombinant protein yield, solubility, and activity, aiding in expression optimization.

 

4. Fungal Metabolism Studies

Analyze protein expression under varying nutrient levels, temperatures, and pH conditions to uncover the physiological and adaptive mechanisms of yeast-like fungi.