MtoZ Biolabs' Tyrosine Protein Kinase Inhibitor is designed to suppress the activity of protein tyrosine phosphatases during the protein extraction from cells and tissue samples. This inhibitor helps minimize tyrosine dephosphorylation during sample handling, thereby preserving authentic phosphorylation signals. It is widely applied in phosphoprotein extraction, sample preservation, and downstream phosphorylation analysis.
Product Overview
During protein extraction and sample processing, the preservation of tyrosine phosphorylation faces several critical challenges:
1. Rapid Dephosphorylation After Cell Lysis
Upon cell or tissue disruption, endogenous phosphatases are rapidly released, and tyrosine dephosphorylation can occur within minutes.
2. Underestimation of True Phosphorylation Levels
Delays in processing, temperature fluctuations, or handling variability can artificially reduce tyrosine phosphorylation signals.
3. Interference with key Signaling Readouts
Tyrosine phosphorylation-dependent pathways, such as RTK and STAT signaling, are highly sensitive to dephosphorylation, leading to distorted biological interpretation.
4. Reduced Reproducibility Across Samples
Variations in sample origin and processing time introduce inconsistent dephosphorylation backgrounds, compromising comparability between experiments.
Advantages
1. Efficient Inhibition of Tyrosine Dephosphorylation
Help maintain tyrosine phosphorylation status during lysis and sample preparation.
2. Improved Signal Fidelity and Comparability
Minimize technical artifacts introduced during sample handling and enhance data consistency.
3. Compatibility with Downstream Analyses
Suitable for Western blotting, immunoprecipitation, and phosphorylation-related analytical workflows.
4. Facilitate Standardized Workflows
Support consistent sample processing conditions and reduce variability caused by handling differences.
Technical Support
1. Protocol Downloads
MtoZ Biolabs provides comprehensive experimental protocols and product manuals to help ensure smooth and reproducible workflows. For access to specific documents or detailed instructions, please contact our support team.
2. Customized Service Support
Our experienced scientists offer tailored technical assistance and customized solutions based on your specific research goals, helping you achieve optimal experimental outcomes.
3. FAQ and Expert Assistance
We provide responsive and professional technical support to address your questions efficiently. Our experts are ready to help you identify and resolve experimental challenges quickly and accurately.
