MtoZ Biolabs' Calpain Inhibitor is specifically designed to inhibit Ca²+-dependent calpain activity, effectively preventing non-specific protein degradation. This product is widely used in protein extraction and proteomics studies to preserve sample integrity and ensure reliable analytical results.
Product Overview
During cell or tissue lysis, proteins are removed from their native cellular environment and become highly susceptible to degradation by endogenous proteases. This issue is particularly pronounced under calcium-containing conditions and is mainly reflected in the following aspects:
1. Upon cell or tissue disruption, endogenous calpains are rapidly activated, leading to non-specific cleavage of target proteins.
2. The presence of Ca²⁺ significantly enhances calpain activity, making it difficult to achieve complete inhibition even at low temperatures.
3. Partial protein degradation compromises molecular weight determination, quantitative accuracy, and experimental reproducibility.
4. Low-abundance or structurally sensitive proteins are especially vulnerable, increasing uncertainty in downstream mass spectrometry analysis.
Advantages
1. The Calpain Inhibitor can efficiently inhibit calpain activity, significantly reducing protein degradation.
2. MtoZ Biolabs' Calpain Inhibitor is suitable for a wide range of sample types, including cells, animal tissues, and plant tissues.
3. The inhibitor is highly compatible with mass spectrometry workflows, without interfering with downstream analyses.
4. The product can be used in combination with protease inhibitor cocktails to enhance overall protein protection.
Technical Support
1. Protocol Downloads
MtoZ Biolabs provides comprehensive experimental protocols and product manuals to help ensure smooth and reproducible workflows. For access to specific documents or detailed instructions, please contact our support team.
2. Customized Service Support
Our experienced scientists offer tailored technical assistance and customized solutions based on your specific research goals, helping you achieve optimal experimental outcomes.
3. FAQ and Expert Assistance
We provide responsive and professional technical support to address your questions efficiently. Our experts are ready to help you identify and resolve experimental challenges quickly and accurately.
