The MtoZ Biolabs' EDTA-Free Protease and Phosphatase Inhibitor Cocktail is specifically designed for protein extraction by inhibiting endogenous proteases and phosphatases, thereby preventing protein degradation while maintaining compatibility with metal-dependent proteins and downstream functional analyses. The inhibitor cocktail is suitable for Western blot analysis, proteomics sample preparation, and post-translational modification studies such as phosphorylation.
Product Overview
During protein extraction, maintaining protein integrity and native biochemical properties is particularly challenging, especially in EDTA-sensitive experimental systems. Common difficulties include:
1. Rapid Activation of Endogenous Proteases
Cell lysis leads to the mediated release and activation of multiple proteases, resulting in non-specific protein degradation that compromises molecular weight determination and quantitative accuracy.
2. Loss of Phosphorylation and Other Modification States
Endogenous phosphatases can rapidly remove phosphorylation marks, disrupting signaling pathway information and affecting downstream mechanistic studies.
3. Edta Incompatibility with Metal-Dependent Proteins and Assays
Conventional inhibitor cocktails containing EDTA may chelate essential metal ions, interfering with metalloprotein stability, enzyme activity assays, and metal-dependent protein complexes.
4. Instability of Low-Abundance and Labile Proteins
Regulatory proteins and transiently modified proteins are particularly vulnerable to degradation or inactivation during extraction.
Advantages
✅ Broad-Spectrum Inhibition
Simultaneous inhibition of serine proteases, cysteine proteases, and multiple classes of phosphatases.
✅ EDTA-Free Formulation
Maintains compatibility with metalloproteins, metal-dependent enzymes, and downstream functional or activity assays.
✅ Ready-to-Use Formula
No additional preparation is required, ensuring simplified workflows and high experimental reproducibility.
✅ High Compatibility
Suitable for Western blot, LC-MS/MS–based proteomics, PTM analysis, and enzyme activity studies.
Technical Support
1. Protocol Downloads
MtoZ Biolabs provides comprehensive experimental protocols and product manuals to help ensure smooth and reproducible workflows. For access to specific documents or detailed instructions, please contact our support team.
2. Customized Service Support
Our experienced scientists offer tailored technical assistance and customized solutions based on your specific research goals, helping you achieve optimal experimental outcomes.
3. FAQ and Expert Assistance
We provide responsive and professional technical support to address your questions efficiently. Our experts are ready to help you identify and resolve experimental challenges quickly and accurately.
