The MtoZ Biolabs' Cysteine Protease Inhibitor is specifically designed to suppress the activity of cysteine proteases. This inhibitor effectively prevents non-specific protein degradation during sample preparation. It is widely applied in protein extraction, sample preservation, and proteomics analysis.
Product Overview
During protein extraction and cell or tissue lysis, if protease activity is not effectively controlled, endogenous proteases are rapidly activated upon lysis, leading to systematic impacts on protein stability and data reliability.
1. Rapid Activation of Endogenous Proteases
Upon cell or tissue lysis, cysteine proteases are quickly released and continuously degrade target proteins.
2. Degradation of Low-Abundance and Unstable Proteins
Key signaling or structural proteins are often cleaved at early stages, leading to detection failure.
3. Severe Interference from Lysosomal Proteases
Under acidic conditions, cathepsin activity is enhanced, significantly compromising protein integrity.
4. Reproducibility Challenges Caused by Sample Variability
Protease activity varies across different tissues or experimental conditions, making consistent results difficult to achieve.
Advantages
✅ High-Efficiency Inhibition
Effectively targets representative cysteine proteases and markedly reduces non-specific protein degradation.
✅ Preservation of Protein Integrity
Helps maintain native molecular weight and post-translational modification information.
✅ Compatibility with Downstream Analyses
Suitable for SDS-PAGE, Western blotting, and mass spectrometry-based applications.
✅ Improved Data Reliability
Minimizes technical bias introduced during sample handling and enhances experimental reproducibility.
Technical Support
1. Protocol Downloads
MtoZ Biolabs provides comprehensive experimental protocols and product manuals to help ensure smooth and reproducible workflows. For access to specific documents or detailed instructions, please contact our support team.
2. Customized Service Support
Our experienced scientists offer tailored technical assistance and customized solutions based on your specific research goals, helping you achieve optimal experimental outcomes.
3. FAQ and Expert Assistance
We provide responsive and professional technical support to address your questions efficiently. Our experts are ready to help you identify and resolve experimental challenges quickly and accurately.
