The MtoZ Biolabs' Endogenous Enzyme Inhibitor (For Cryosections) is specifically designed to suppress endogenous enzymatic activities in frozen tissue sections. This inhibitor effectively reduces background interference while preserving antigen signals and is widely used in frozen-section IHC and IF analyses.
Product Overview
During cryosection handling and subsequent staining procedures, endogenous enzymes within tissues are readily activated under non-physiological conditions. These enzymes represent major sources of elevated background and signal distortion, mainly manifested as follows:
1. High Endogenous Peroxidase Activity
Blood cells and myeloid cells in frozen sections are rich in peroxidase activity, which can generate strong non-specific background in HRP-based detection systems.
2. Interference from Endogenous Alkaline Phosphatase
Tissues such as intestine, kidney, and bone exhibit high alkaline phosphatase activity, compromising staining specificity in AP-based detection systems.
3. Loss of Phosphorylation Signals Caused by Endogenous Phosphatases
Dephosphorylation during processing and incubation leads to reduced or distorted signals when detecting phosphorylated antigens.
4. Enzyme Activation During Thawing and Handling
Enzymatic activities released during cryosection thawing and washing steps amplify background noise and reduce signal clarity.
Advantages
✅ Multi-Target Inhibition Strategy
Simultaneously suppresses peroxidases, alkaline phosphatases, and other endogenous enzymatic activities.
✅ Significant Reduction of Non-Specific Background
Improves signal-to-noise ratio and enhances reproducibility in IHC and IF analyses.
✅ Compatible with Multiple Staining Systems
Suitable for HRP-, AP-, and phosphorylation-specific antibody-based workflows.
✅ Ready-to-Use and Easy to Operate
No additional preparation required, enabling standardized and streamlined experimental procedures.
Technical Support
1. Protocol Downloads
MtoZ Biolabs provides comprehensive experimental protocols and product manuals to help ensure smooth and reproducible workflows. For access to specific documents or detailed instructions, please contact our support team.
2. Customized Service Support
Our experienced scientists offer tailored technical assistance and customized solutions based on your specific research goals, helping you achieve optimal experimental outcomes.
3. FAQ and Expert Assistance
We provide responsive and professional technical support to address your questions efficiently. Our experts are ready to help you identify and resolve experimental challenges quickly and accurately.
