MtoZ Biolabs' Protease Inhibitor is supplied as a ready-to-use, broad-spectrum formulation that effectively prevents protein degradation in a single step, thereby ensuring comprehensive protection of protein samples.
Product Overview
During protein extraction and cell lysis, endogenous proteases can be rapidly released and degrade target proteins, potentially compromising experimental outcomes:
1. Rapid Protein Degradation
Upon sample removal or lysis, diverse classes of proteases, including serine, cysteine, aspartic, and metalloproteases, rapidly attack and degrade target proteins.
2. Deviation of Experimental Results
Degradation alters Western blot banding patterns, interferes with interaction analysis in co-immunoprecipitation assays, and leads to inaccurate mass spectrometric quantification, ultimately giving rise to misleading biological interpretations.
3. Labor-Intensive and Hazardous Self-Preparation
Purchasing and preparing multiple inhibitors individually is costly, procedurally complex, and difficult to optimize; in addition, some inhibitors (e.g., PMSF) display high toxicity and low stability, posing safety concerns.
4. Incomplete Inhibition Spectrum
A single inhibitor (e.g., PMSF alone) targets only specific protease families and cannot ensure comprehensive sample protection.
Advantages
✅ Broad-Spectrum and Efficient
MtoZ Biolabs' Protease Inhibitor incorporates multiple potent inhibitory compounds in an optimized formulation, enabling simultaneous inhibition of major endogenous protease families, including serine, cysteine, aspartic, and metalloproteases, to ensure comprehensive protease suppression.
✅ Convenient, Safe, and Stable
Supplied as a premixed solution requiring only direct addition to lysis buffers, eliminating preparation complexity and associated risks, while offering excellent stability for routine handling and storage.
✅ Excellent Compatibility
Fully compatible with commonly used lysis buffers (e.g., RIPA, NP-40, homogenization buffers) and does not interfere with downstream protein quantification, electrophoresis, immunoprecipitation, or mass spectrometric workflows.
✅ Preservation of Modification Information
By preventing proteolytic degradation, the kit helps maintain native post-translational modification profiles, ensuring accuracy in proteomic and modification-focused studies.
Technical Support
1. Protocol Downloads
MtoZ Biolabs provides comprehensive experimental protocols and product manuals to help ensure smooth and reproducible workflows. For access to specific documents or detailed instructions, please contact our support team.
2. Customized Service Support
Our experienced scientists offer tailored technical assistance and customized solutions based on your specific research goals, helping you achieve optimal experimental outcomes.
3. FAQ and Expert Assistance
We provide responsive and professional technical support to address your questions efficiently. Our experts are ready to help you identify and resolve experimental challenges quickly and accurately.
