The MtoZ Biolabs' Pepsin Inhibitor is specifically designed for efficient suppression of pepsin activity. This inhibitor is widely used in protein extraction and proteomics studies to preserve the integrity of target proteins.
Product Overview
During practical sample handling and cell or tissue lysis, especially when acidic conditions or digestion-related samples are involved, protein extraction often faces multiple challenges that directly affect protein stability and downstream analytical quality.
1. Persistent Enzyme Activity under Acidic Conditions
Pepsin remains highly active at low pH, leading to rapid and nonspecific protein hydrolysis.
2. Loss of Low-Abundance Proteins
Target proteins are prone to early degradation during extraction, compromising detection sensitivity and quantitative accuracy.
3. Instability of Protein Structure and Modifications
Native conformations and PTM sites are easily disrupted, reducing downstream analytical resolution.
4. High Sample Variability
Pepsin levels vary significantly in gastric tissues and digestive fluids, increasing the difficulty of effective inhibition.
Advantages
1. High Selectivity
Precisely inhibit pepsin while minimizing interference with other proteins and enzymatic systems.
2. Rapid and Stable Performance
Act quickly and reliably under acidic conditions to protect proteins throughout the extraction process.
3. Compatible with Downstream Analyses
Does not interfere with SDS-PAGE, LC-MS/MS, or quantitative proteomics workflows.
4. Improved Data Quality
Effectively reduce background degradation, enhancing reproducibility and interpretability of results.
Technical Support
1. Protocol Downloads
MtoZ Biolabs provides comprehensive experimental protocols and product manuals to help ensure smooth and reproducible workflows. For access to specific documents or detailed instructions, please contact our support team.
2. Customized Service Support
Our experienced scientists offer tailored technical assistance and customized solutions based on your specific research goals, helping you achieve optimal experimental outcomes.
3. FAQ and Expert Assistance
We provide responsive and professional technical support to address your questions efficiently. Our experts are ready to help you identify and resolve experimental challenges quickly and accurately.
