The Transmembrane Protein Extraction Kit from MtoZ Biolabs is designed to efficiently isolate transmembrane proteins by addressing their strong membrane association and hydrophobic characteristics, providing high-quality material for membrane protein characterization and downstream analytical workflows.
Product Overview
1. Tight Association with the Lipid Bilayer
Transmembrane proteins are embedded within the lipid bilayer, and insufficient solubilization often leads to incomplete extraction and low recovery.
2. Low Abundance Relative to Soluble Proteins
Membrane proteins typically occur at lower abundance than cytosolic proteins. Non-selective extraction can introduce large amounts of soluble proteins, reducing the visibility of membrane targets in downstream analyses.
3. Detergent Interference in Downstream Workflows
Certain strong detergents may disrupt membrane protein structure or interfere with mass spectrometry, immunoassays, and functional studies.
Advantages
The MtoZ Biolabs Transmembrane Protein Extraction Kit offers the following benefits:
✅ Efficient Solubilization of Hydrophobic Proteins
The optimized detergent system enables effective membrane disruption and solubilization, improving recovery of transmembrane proteins.
✅ High-Purity Membrane Fractions
The extraction workflow reduces co-extraction of cytosolic components, generating cleaner membrane protein preparations and supporting more reliable data interpretation.
✅ Compatibility with Downstream Applications
The detergent composition is selected to support downstream use in mass spectrometry, Western blotting, immunoprecipitation, and functional assays.
✅ Broad Applicability Across Cell Types
Compatible with mammalian, microbial, and plant samples, the kit supports membrane protein studies across research areas such as receptor biology, transporter function, and signaling pathway analysis.
Technical Support
1. Protocol Downloads
MtoZ Biolabs provides comprehensive experimental protocols and product manuals to help ensure smooth and reproducible workflows. For access to specific documents or detailed instructions, please contact our support team.
2. Customized Service Support
Our experienced scientists offer tailored technical assistance and customized solutions based on your specific research goals, helping you achieve optimal experimental outcomes.
3. FAQ and Expert Assistance
We provide responsive and professional technical support to address your questions efficiently. Our experts are ready to help you identify and resolve experimental challenges quickly and accurately.
