The Cell Culture Supernatant Protein Extraction Kit from MtoZ Biolabs is designed for efficient protein extraction from cell culture supernatants, addressing challenges like low-abundance proteins and interference from media components, making it suitable for extracellular protein analysis and secretome studies.
Product Overview
When extracting proteins from cell culture supernatants, researchers often face the following challenges:
1. Low-Abundance Proteins
High-abundance proteins in the supernatant can mask low-abundance proteins, making it difficult to detect and quantify them accurately.
2. Interference from Culture Media Components
Components such as salts, lipids, and small molecules from the culture media can co-extract with proteins, reducing sample purity and hindering accurate downstream analysis, particularly in mass spectrometry and immunoassays.
3. Protein Stability
Proteins in cell culture supernatants can degrade due to protease activity or unstable conditions, affecting the integrity of the sample for subsequent analyses.
MtoZ Biolabs' Cell Culture Supernatant Protein Extraction Kit addresses these challenges with an optimized extraction protocol that improves recovery of low-abundance proteins, reduces contamination from media components, and stabilizes proteins to ensure high-quality samples for further analysis.
Advantages
The MtoZ Biolabs Cell Culture Supernatant Protein Extraction Kit offers the following benefits:
✅ Improved Protein Recovery
The optimized extraction buffer ensures efficient recovery of both high- and low-abundance proteins from cell culture supernatants.
✅ Reduced Contamination
Designed to selectively extract proteins, the kit minimizes the co-extraction of unwanted media components, ensuring cleaner samples for accurate analysis.
✅ Protein Stabilization
Built-in protease inhibitors and stabilizing agents help prevent protein degradation during extraction, maintaining sample integrity.
✅ Compatibility with Various Cell Types and Media
The kit is suitable for use with a wide range of cell types and culture media, making it versatile for extracellular protein analysis, secretome profiling, and related research applications.
Technical Support
1. Protocol Downloads
MtoZ Biolabs provides comprehensive experimental protocols and product manuals to help ensure smooth and reproducible workflows. For access to specific documents or detailed instructions, please contact our support team.
2. Customized Service Support
Our experienced scientists offer tailored technical assistance and customized solutions based on your specific research goals, helping you achieve optimal experimental outcomes.
3. FAQ and Expert Assistance
We provide responsive and professional technical support to address your questions efficiently. Our experts are ready to help you identify and resolve experimental challenges quickly and accurately.
