The Cell Histone Extraction Kit from MtoZ Biolabs is developed to efficiently isolate core histones and associated chromatin-bound proteins from cultured cells, providing high-quality material for epigenetic, chromatin structure, and PTM-focused studies.
Product Overview
Histone extraction from cells presents several technical challenges due to the tightly organized nature of chromatin:
1. Strong DNA-Protein Interactions
Histones are tightly bound to DNA within nucleosomes. Without proper extraction conditions, histones remain embedded in chromatin, resulting in low recovery and incomplete representation of H2A, H2B, H3, and H4.
2. High Sensitivity to Extraction Conditions
Histone proteins carry extensive post-translational modifications, including acetylation, methylation, phosphorylation, and ubiquitination. Suboptimal extraction can cause loss or alteration of these modifications, affecting downstream epigenetic analysis.
3. Contamination from Non-Chromatin Proteins
General cell lysis releases cytosolic and nuclear matrix proteins that can dilute or obscure histone signals, reducing data accuracy in mass spectrometry and immunoblotting assays.
The MtoZ Biolabs Cell Histone Extraction Kit overcomes these challenges by using a stepwise extraction process designed to release histones efficiently while maintaining the stability of histone modifications. This results in high-purity histone preparations suitable for various epigenetic studies.
Advantages
The MtoZ Biolabs Cell Histone Extraction Kit offers the following advantages:
✅ Efficient Histone Extraction
The kit's optimized protocol ensures effective extraction of core histones (H2A, H2B, H3, H4) and chromatin-bound proteins, achieving high yield and minimal loss of material.
✅ Preservation of Histone Modifications
The extraction process is designed to maintain labile post-translational modifications on histones, ensuring that acetylation, methylation, phosphorylation, and other PTMs remain intact for accurate epigenetic analysis.
✅ High Purity for Accurate Analysis
The kit minimizes contamination from non-histone proteins, reducing background noise in protein samples and providing cleaner material for techniques like mass spectrometry, Western blotting, and chromatin immunoprecipitation (ChIP).
✅ Versatility Across Cell Types
Compatible with a broad range of cell types, including mammalian cell lines, primary cells, and suspension cultures, the kit is suitable for a wide variety of epigenetic and chromatin-related research applications.
Technical Support
1. Protocol Downloads
MtoZ Biolabs provides comprehensive experimental protocols and product manuals to help ensure smooth and reproducible workflows. For access to specific documents or detailed instructions, please contact our support team.
2. Customized Service Support
Our experienced scientists offer tailored technical assistance and customized solutions based on your specific research goals, helping you achieve optimal experimental outcomes.
3. FAQ and Expert Assistance
We provide responsive and professional technical support to address your questions efficiently. Our experts are ready to help you identify and resolve experimental challenges quickly and accurately.
