The Insoluble Protein Extraction Kit from MtoZ Biolabs is designed to efficiently extract and solubilize proteins from insoluble cellular fractions, enabling the analysis of challenging proteins that are typically found in inclusion bodies or poorly soluble aggregates.
Product Overview
Extracting insoluble proteins from cellular aggregates or inclusion bodies is inherently challenging due to their poor solubility and aggregation:
1. Low Solubility of Insoluble Proteins
Proteins that form insoluble aggregates, such as those expressed in recombinant systems, are difficult to solubilize with standard extraction methods, resulting in poor protein recovery and low yields.
2. Protein Aggregation and Precipitation
Many proteins, particularly those overexpressed in bacteria or other systems, tend to aggregate into insoluble structures, making them less accessible for conventional extraction methods and reducing the efficiency of downstream assays.
3. Denaturation Risk During Extraction
Harsh extraction conditions can lead to protein denaturation, loss of functional activity, and changes to the protein's tertiary structure, especially for proteins prone to aggregation.
The MtoZ Biolabs Insoluble Protein Extraction Kit provides a specialized extraction process that effectively solubilizes aggregated or poorly soluble proteins while minimizing denaturation, making it ideal for proteins in inclusion bodies or other insoluble fractions.
Advantages
The MtoZ Biolabs Insoluble Protein Extraction Kit offers the following benefits:
✅ Efficient Solubilization of Aggregated Proteins
The optimized buffer system effectively solubilizes proteins from inclusion bodies and aggregated fractions, ensuring high recovery and minimal loss of material, even from challenging proteins.
✅ Prevention of Protein Denaturation
The extraction protocol is designed to minimize the risk of protein denaturation, preserving the native structure and functional activity of the solubilized proteins for downstream applications.
✅ High Purity and Low Background
The kit minimizes the co-extraction of unwanted soluble proteins and cellular debris, providing purified proteins that are ideal for sensitive applications such as mass spectrometry, Western blotting, and enzyme activity assays.
✅ Compatibility with Multiple Expression Systems
The kit is suitable for use with a wide range of expression systems, including bacterial (E. coli), yeast, and mammalian cells, making it versatile for recombinant protein research, structural studies, and functional analysis of insoluble proteins.
Technical Support
1. Protocol Downloads
MtoZ Biolabs provides comprehensive experimental protocols and product manuals to help ensure smooth and reproducible workflows. For access to specific documents or detailed instructions, please contact our support team.
2. Customized Service Support
Our experienced scientists offer tailored technical assistance and customized solutions based on your specific research goals, helping you achieve optimal experimental outcomes.
3. FAQ and Expert Assistance
We provide responsive and professional technical support to address your questions efficiently. Our experts are ready to help you identify and resolve experimental challenges quickly and accurately.
