The Membrane, Cytoplasmic, and Nuclear Proteins Stepwise Extraction Kit from MtoZ Biolabs provides a structured workflow for sequential isolation of cytoplasmic, membrane-associated, and nuclear proteins, supporting accurate compartment-specific proteomic and biochemical studies.
Product Overview
Subcellular protein studies require clear separation of cytoplasmic, membrane-associated, and nuclear proteins to accurately assess compartment-specific signaling, transport processes, and regulatory mechanisms. However, researchers frequently encounter challenges during fractionation, such as:
1. Overlap Between Soluble and Membrane-Associated Proteins
Non-selective extraction may release cytoplasmic proteins together with peripheral and integral membrane proteins, reducing clarity when studying receptor signaling, transporter function, or membrane-associated pathways.
2. Limited Enrichment of Nuclear Proteins
Many nuclear proteins are tightly associated with chromatin or the nuclear matrix. Without targeted extraction conditions, nuclear fractions may contain cytoplasmic or membrane proteins, complicating epigenetic and structural analyses.
3. Distinct Extraction Requirements Across Compartments
Cytoplasmic, membrane, and nuclear proteins differ in solubility, biochemical stability, and structural context. A single extraction buffer cannot efficiently recover all three groups while maintaining recovery and protein integrity.
The Membrane, Cytoplasmic, and Nuclear Proteins Stepwise Extraction Kit from MtoZ Biolabs applies a compartment-specific extraction strategy that yields clearly separated fractions with minimal cross-contamination. Each step uses a dedicated buffer optimized for the physicochemical properties of its target protein group.
Advantages
The MtoZ Biolabs Membrane, Cytoplasmic, and Nuclear Proteins Stepwise Extraction Kit provides:
✅ Defined Subcellular Fractionation
Sequential extraction produces distinct cytoplasmic, membrane-associated, and nuclear protein fractions for precise compartment-resolved analysis.
✅ Optimized Buffers for Each Protein Class
Tailored formulations enhance solubility and recovery of soluble cytoplasmic proteins, lipid-associated membrane proteins, and chromatin- or matrix-bound nuclear proteins.
✅ High Purity for Downstream Workflows
Clean fractions reduce background noise and improve performance in mass spectrometry, Western blotting, receptor profiling, interaction studies, and nuclear protein analysis.
✅ Broad Compatibility Across Cell Types
Suitable for mammalian cell lines, primary cells, and selected plant or microbial systems, enabling diverse applications in signaling research, membrane biology, nuclear function, and systems-level proteomics.
Technical Support
1. Protocol Downloads
MtoZ Biolabs provides comprehensive experimental protocols and product manuals to help ensure smooth and reproducible workflows. For access to specific documents or detailed instructions, please contact our support team.
2. Customized Service Support
Our experienced scientists offer tailored technical assistance and customized solutions based on your specific research goals, helping you achieve optimal experimental outcomes.
3. FAQ and Expert Assistance
We provide responsive and professional technical support to address your questions efficiently. Our experts are ready to help you identify and resolve experimental challenges quickly and accurately.
