The Membrane, Cytoplasmic, and Nuclear Membrane Proteins Stepwise Extraction Kit from MtoZ Biolabs is designed to sequentially isolate cytoplasmic proteins, plasma membrane proteins, and nuclear membrane proteins in a controlled, phase-by-phase workflow, providing high-purity fractions for advanced proteomic and cell biology research.
Product Overview
Obtaining clean and well-separated protein fractions from different cellular compartments requires a workflow capable of resolving structural complexity and minimizing cross-contamination. Stepwise extraction is particularly important for studies that require accurate compartment-specific protein profiling. Key challenges include:
1. Compartment Overlap
Cytoplasmic, plasma membrane, and nuclear membrane proteins vary in solubility and structural properties. Without selective extraction, cross-contamination decreases the reliability of compartment-specific datasets.
2. Different Solubilization Requirements
Lipid-rich plasma and nuclear membranes require tailored detergent systems for efficient extraction, while cytoplasmic proteins must remain intact during the initial extraction step.
3. Impact on Downstream Applications
Mixed fractions introduce high background signals that interfere with mass spectrometry, immunoblotting, and protein interaction studies.
Advantages
The MtoZ Biolabs Membrane, Cytoplasmic, and Nuclear Membrane Proteins Stepwise Extraction Kit provides the following benefits:
✅ Clear Separation of Subcellular Fractions
Sequential extraction produces distinct cytoplasmic, plasma membrane, and nuclear membrane fractions with reduced cross-contamination.
✅ Optimized Buffers for Each Compartment
Each extraction stage uses a buffer formulation suited to the solubility and biochemical properties of its target protein group.
✅ High Purity for Sensitive Analyses
Clean, compartment-specific fractions enhance performance in mass spectrometry, Western blotting, receptor studies, nuclear envelope research, and signaling pathway analysis.
✅ Broad Applicability Across Cell Types
Compatible with mammalian cell lines, primary cells, and selected microbial and plant samples, supporting diverse proteomic and cell biology workflows.
Technical Support
1. Protocol Downloads
MtoZ Biolabs provides comprehensive experimental protocols and product manuals to help ensure smooth and reproducible workflows. For access to specific documents or detailed instructions, please contact our support team.
2. Customized Service Support
Our experienced scientists offer tailored technical assistance and customized solutions based on your specific research goals, helping you achieve optimal experimental outcomes.
3. FAQ and Expert Assistance
We provide responsive and professional technical support to address your questions efficiently. Our experts are ready to help you identify and resolve experimental challenges quickly and accurately.
