The Plasma Membrane Protein Extraction Kit from MtoZ Biolabs is designed to selectively isolate plasma membrane proteins, including transmembrane, lipid-anchored, and peripheral proteins, providing enriched fractions suitable for biochemical and proteomic analysis.
Product Overview
Plasma membrane protein extraction requires addressing several technical challenges:
1. Diverse Modes of Membrane Association
Plasma membrane proteins include multiple structural classes such as transmembrane, lipid-anchored, and peripheral proteins. Each responds differently to extraction conditions, and incomplete solubilization can lead to poor representation of key targets.
2. Low Abundance Compared with Cytosolic Proteins
Plasma membrane proteins are typically less abundant. Direct lysis releases large amounts of soluble proteins that can overshadow membrane proteins and reduce detection sensitivity in downstream assays.
3. Sensitivity to Extraction Reagents
Harsh detergents can disrupt protein conformation or interfere with mass spectrometry, immunoblotting, and receptor-binding experiments.
Advantages
The MtoZ Biolabs Plasma Membrane Protein Extraction Kit offers the following benefits:
✅ Targeted Enrichment of Plasma Membrane Proteins
The workflow isolates membrane-rich fractions using differential extraction, reducing background from cytosolic and organelle proteins.
✅ Optimized Solubilization of Membrane Structures
The carefully formulated detergent system efficiently solubilizes hydrophobic transmembrane proteins while preserving the integrity of lipid-anchored and peripheral proteins.
✅ High Purity for Sensitive Analytical Applications
Reduced contamination enhances suitability for mass spectrometry, membrane proteomics, receptor analysis, transporter studies, and immunodetection assays.
✅ Compatibility with Multiple Cell Types
The kit supports extraction from mammalian cells, primary cells, and selected microbial and plant samples, enabling broad use in studies of signaling pathways, membrane transport, and cell-surface protein biology.
Technical Support
1. Protocol Downloads
MtoZ Biolabs provides comprehensive experimental protocols and product manuals to help ensure smooth and reproducible workflows. For access to specific documents or detailed instructions, please contact our support team.
2. Customized Service Support
Our experienced scientists offer tailored technical assistance and customized solutions based on your specific research goals, helping you achieve optimal experimental outcomes.
3. FAQ and Expert Assistance
We provide responsive and professional technical support to address your questions efficiently. Our experts are ready to help you identify and resolve experimental challenges quickly and accurately.
