The MtoZ Biolabs' Protease and Phosphatase Inhibitor Cocktail is specifically designed for protein extraction to effectively suppress endogenous protease and phosphatase activities during sample preparation, thereby preventing protein degradation and preserving protein integrity. This inhibitor cocktail is suitable for Western blot analysis, proteomics sample preparation, and post-translational modification studies.
Product Overview
During protein extraction, both protein integrity and post-translational modification status are highly susceptible to interference from endogenous enzymatic activities, mainly including the following challenges:
1. Rapid Activation of Endogenous Proteases
Upon cell lysis, multiple proteases are instantly released and activated, leading to non-specific degradation of target proteins and compromising molecular weight determination and quantitative accuracy.
2. Loss of Modification States Caused by Phosphatases
Endogenous phosphatases can rapidly remove phosphorylation modifications, resulting in distorted signaling information and interference with downstream functional analyses.
3. Complex Sample Backgrounds and Diverse Enzyme Profiles
Protease and phosphatase compositions vary significantly across different tissues and species, making it difficult for a single inhibitor to provide comprehensive protection.
4. Instability of Low-Abundance and Labile Proteins
Regulatory proteins and transiently modified proteins exhibit limited stability and are more prone to degradation or inactivation during extraction.
Advantages
✅ Broad-Spectrum Inhibition
Simultaneous inhibition of serine proteases, cysteine proteases, and multiple classes of phosphatases.
✅ Ready-to-Use Formula
No additional preparation is required, enabling simplified workflows and high experimental reproducibility.
✅ High Compatibility
AEBSF-free and compatible with Western blot, LC-MS/MS, and PTM-related analyses.
Technical Support
1. Protocol Downloads
MtoZ Biolabs provides comprehensive experimental protocols and product manuals to help ensure smooth and reproducible workflows. For access to specific documents or detailed instructions, please contact our support team.
2. Customized Service Support
Our experienced scientists offer tailored technical assistance and customized solutions based on your specific research goals, helping you achieve optimal experimental outcomes.
3. FAQ and Expert Assistance
We provide responsive and professional technical support to address your questions efficiently. Our experts are ready to help you identify and resolve experimental challenges quickly and accurately.
