MtoZ Biolabs' Phosphatase Inhibitor is composed of multiple active components targeting both Ser/Thr and Tyr phosphatases. This formulation rapidly suppresses endogenous phosphatase activity during sample handling, preserving native phosphorylation states to the greatest extent possible. The inhibitor is suitable for phospho-Western blotting, phospho-specific antibody detection, IF, IHC, pull-down assays, immunoprecipitation (IP/Co-IP), and phosphopeptide mass spectrometry analysis.
Product Overview
Maintaining native protein phosphorylation levels becomes particularly challenging during in vitro sample processing, where non-specific dephosphorylation readily occurs. The main challenges include:
1. Rapid Loss of Phosphorylation
Upon cell lysis, multiple Ser/Thr and Tyr phosphatases are instantly activated, causing key phosphorylation sites to disappear within minutes and compromising accurate signaling assessments.
2. High Abundance of Endogenous Phosphatases
Crude lysates contain numerous potent phosphatases capable of altering protein modification states, resulting in deviations from true cellular phosphorylation levels.
3. Increased Instability During Handling
Temperature fluctuations, mixing, or prolonged lysis steps accelerate dephosphorylation and reduce reproducibility across experiments.
4. Distortion of Downstream Analyses
Loss of phosphorylation leads to weakened Western blot signals, unreliable phospho-antibody detection, and significant bias in phosphoproteomic identification and quantification.
5. Difficulty Preserving Native Regulatory States
Without effective inhibition, samples cannot maintain their instantaneous cellular phosphorylation levels, hindering accurate evaluation of kinase activity and signaling dynamics.
Advantages
✅ High Efficiency
A composite Ser/Thr and Tyr phosphatase inhibitor system rapidly blocks endogenous phosphatase activity and stabilizes phosphorylation sites.
✅ Preservation
Effectively prevents non-specific dephosphorylation during lysis and handling, maintaining phosphorylation levels closer to in-cell conditions.
✅ Compatibility
Suitable for Western blotting, IF, IP/Co-IP, and phosphopeptide mass spectrometry, ensuring reliable qualitative and quantitative outcomes.
Technical Support
1. Protocol Downloads
MtoZ Biolabs provides comprehensive experimental protocols and product manuals to help ensure smooth and reproducible workflows. For access to specific documents or detailed instructions, please contact our support team.
2. Customized Service Support
Our experienced scientists offer tailored technical assistance and customized solutions based on your specific research goals, helping you achieve optimal experimental outcomes.
3. FAQ and Expert Assistance
We provide responsive and professional technical support to address your questions efficiently. Our experts are ready to help you identify and resolve experimental challenges quickly and accurately.
