• IP-MS Validated LMX1B Polyclonal Antibody
    • Cat#: MSVAB-13251
    • Primary Applications: IP, Co-IP, IP-MS
    • Species Reactivity: Human, Mouse, Rat
    • Antibody Specificity: LMX1B
    • Other Applications: WB, IF, ELISA, IHC, ChIP

    Customer Support Program

    • First-time antibody customers may receive IP-MS identification for one sample at no additional cost with their first antibody purchase.
    • Customers who purchase antibodies may receive 10% off subsequent IP-MS identification services.
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Target Information

Target informationLIM homeobox transcription factor 1 beta (LMX1B), also known as LIM-HD transcription factor 1B, is a LIM-homeodomain protein that functions in transcriptional regulation during development and cell fate specification. As a DNA-binding regulatory factor, LMX1B is frequently studied for its roles in gene expression control, tissue patterning, and differentiation, making it a relevant target in developmental biology and transcription-focused research. The IP-MS Validated LMX1B Polyclonal Antibody from MtoZ Biolabs is an IP-MS validated antibody developed for selective enrichment of LMX1B in proteomic studies. This IP-MS validated LMX1B Polyclonal Antibody supports efficient immunoprecipitation prior to downstream mass spectrometry analysis, providing a reliable LMX1B antibody validated by IP-MS for target-centered workflows. Researchers can use this LMX1B IP antibody and LMX1B antibody for immunoprecipitation in IP-MS workflows to facilitate confident detection and characterization of LMX1B-associated protein signals.
Target nameLIM homeobox transcription factor 1, beta
AliasesLIM homeobox transcription factor 1-beta, LMX 1.2, LMX1, LMX1.2, LMX-1.2
UniProt accessionO60663

Product Introduction

Primary applications
  • IP (Immunoprecipitation)
  • Co-IP (Co-Immunoprecipitation)
  • IP-MS (Immunoprecipitation-Mass Spectrometry)

Before delivery, each antibody undergoes IP-MS validation to verify its ability to recognize and enrich the intended target protein

Other applicationsWB, IF, ELISA, IHC, ChIP
Species reactivityHuman, Mouse, Rat
ClonalityPolyclonal
HostRabbit
Recommended dilution ratioWB : 1:1000-1:6000
Specification50 μL,100 μL,150 μL
Storage conditionShort-term storage at 2-8 °C. Long-term storage at -20 °C. Avoid repeated freeze-thaw cycles

MtoZ Biolabs IP-MS Workflow Products

MtoZ Biolabs offers a fully integrated IP-MS workflow solution, combining antibodies validated for IP-MS with expertly paired IP kits and seamless downstream mass spectrometry services. Our platform empowers researchers to achieve reliable target enrichment, protein interaction mapping, and comprehensive proteomic profiling with confidence and efficiency.

IP-MS Validated Antibodies

  • Antibodies rigorously tested for IP-MS applications
  • Minimize experimental uncertainty and accelerate research timelines
  • Broad applicability across multiple species and protein targets

Expertly Paired IP Kits

  • Designed and validated to complement MtoZ IP-MS antibodies
  • Detailed step-by-step experimental protocols for reproducible target enrichment
  • Optimized workflows to maximize antibody performance

Comprehensive IP-MS Analysis Services

  • Submit immunoprecipitated samples directly to MtoZ Biolabs
  • High-resolution mass spectrometry for target verification, interaction mapping, and proteomics studies
  • Structured, publication-ready reports for seamless documentation and data reproducibility

Antibody Validation Levels

To support different research goals, budgets, and evidence requirements, our antibodies are available with multiple validation levels. Each antibody undergoes IP-MS validation before delivery to confirm target-specific recognition and enrichment. For projects requiring additional confirmation, Western blot validation can be added to provide orthogonal evidence of target detection.

Validation levelIncluded validationBest for
IP-MS OnlyConfirms target-specific enrichment by immunoprecipitation followed by mass spectrometry analysis.IP-based protein enrichment and target specificity assessment.
Western Blot OnlyConfirms target detection by Western blot under defined experimental conditions.Routine target detection and expression-level assessment.
IP-MS + Western BlotCombines mass spectrometry-based target enrichment evidence with Western blot-based detection evidence.Customers requiring more comprehensive validation support.

Antibody Validation Workflow

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Validation Report Included

IP-MS

  • IP experiment record
  • Antibody usage information
  • Original experimental image
  • Mass spectrometry parameters
  • Identified target peptides
  • Representative MS/MS spectra

Western Blot

  • WB experimental conditions
  • Original blot image
  • Qualitative target band recognition result

MtoZ Biolabs IP-MS Antibody Solutions

Traditional Antibody Supplier

Conventional workflow
  • Customers need to purchase small amounts of antibody for in-house testing

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  • Validation depends on customer-provided samples and workflows

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  • Suppliers usually stop support after product delivery

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  • Customers must find a separate provider for MS or interaction analysis

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  • Higher risk of repeated trial-and-error

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MtoZ Biolabs IP-MS Antibody Solutions

Recommended
  • MtoZ Biolabs can provide IP-MS validation before or at the time of purchase

    01
  • Validation is supported by MS-based evidence and a detailed report

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  • Antibody purchase can be directly connected to downstream IP-MS services

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  • Antibody and downstream IP-MS analysis can be completed within a single workflow

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  • Reduces trial attempts and accelerates project progress

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Why Choose MtoZ Biolabs?

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Antibodies Validated

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Target Proteins

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Research Institutions Served

Full Validation Reports Included

Structured IP/Co-IP/IP-MS validation reports are included with every antibody for easy lab recordkeeping and project documentation.

Ultra-High Resolution MS Platform

IP-MS validation on high-resolution LC-MS/MS instrumentation for confident target enrichment and specificity assessment.

Complementary Protein Interaction Analysis Services

In addition to IP-MS validated antibodies, MtoZ Biolabs offers supporting interaction analysis services for downstream protein enrichment and protein interaction studies.

FAQ

IP-MS validation means that the antibody is first used to pull down proteins from a cell lysate, and the captured proteins are then analyzed by mass spectrometry. This helps confirm whether the antibody can recognize and enrich its intended target protein, instead of mainly pulling down unrelated background proteins.
IP-MS validation does not require a long additional wait. Based on our optimized workflow, it typically adds no more than 5 to 7 business days to the standard processing time. After validation is completed, the antibody and validation report are delivered together.
The validation report includes key experimental records, workflow details, antibody usage information, mass spectrometry settings, target protein identification results, and signal intensity evaluation. The report provides clear supporting data that customers can review and use for project documentation.
We use an ultra-high resolution mass spectrometry platform for IP-MS validation. This platform supports accurate protein identification and provides reliable peptide-level evidence for reviewing antibody validation results.
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